BMRBj - BMREntryMaster

Found 1 Document (1.605 seconds)

BMRB: 4996

1IIO

NMR-Based Structure of the Conserved Protein MTH865 from the Archea Methanobacterium thermoautotrophicum

Authors

Lee, G.M., Edwards, A.M., Arrowsmith, C.H., McIntosh, L.P.

Assembly

MTH865

Entity

1. MTH865 (polymer, Thiol state: all free), 84 monomers, 8673.657 Da Detail

GSHMKMGVKE DIRGQIIGAL AGADFPINSP EELMAALPNG PDTTCKSGDV ELKASDAGQV LTADDFPFKS AEEVADTIVN KAGL

Formula weight

8673.657 Da

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 95.1 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.1 % (853 of 897)	95.5 % (441 of 462)	94.0 % (330 of 351)	97.6 % (82 of 84)
Backbone	94.7 % (468 of 494)	94.8 % (163 of 172)	93.8 % (228 of 243)	97.5 % (77 of 79)
Sidechain	96.0 % (459 of 478)	95.9 % (278 of 290)	96.2 % (176 of 183)	100.0 % (5 of 5)
Aromatic	88.2 % (30 of 34)	88.2 % (15 of 17)	88.2 % (15 of 17)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. Methanobacterium thermoautotrophicum open reading frame 865

GSHMKMGVKE DIRGQIIGAL AGADFPINSP EELMAALPNG PDTTCKSGDV ELKASDAGQV LTADDFPFKS AEEVADTIVN KAGL

Show sample condition

Sample #1

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 15N]		0.5 mM

Sample #2

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 13C; U-100% 15N]		0.5 mM

Sample #3

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Methanobacterium thermoautotrophicum open reading frame 865	[U-10% 13C; U-100% 15N]		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.28 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1IIO, Strand ID: A Detail

Release date

2001-10-07

Citation

Letter to the Editor: NMR-Based Structure of the Conserved Protein MTH865 from the Archaeon Methanobacterium thermoautotrophicum
Lee, G.M., Edwards, A.M., Arrowsmith, C.H., McIntosh, L.P.
J. Biomol. NMR (2001), 21, 63-66, PubMed , DOI:

Related entities 1. MTH865, : 1 : 4 entities Detail

Experiments performed 3 experiments Detail

1 500MHz Unity housed at Univ. of British Columbia

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 15N]		0.5 mM

Sample #2

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 13C; U-100% 15N]		0.5 mM

Sample #3

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Methanobacterium thermoautotrophicum open reading frame 865	[U-10% 13C; U-100% 15N]		0.5 mM

2 600MHz INOVA housed at Univ. of Toronto

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 15N]		0.5 mM

Sample #2

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 13C; U-100% 15N]		0.5 mM

Sample #3

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Methanobacterium thermoautotrophicum open reading frame 865	[U-10% 13C; U-100% 15N]		0.5 mM

3 800MHz INOVA housed at Univ. of Alberta

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 15N]		0.5 mM

Sample #2

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
2	Methanobacterium thermoautotrophicum open reading frame 865	[U-100% 13C; U-100% 15N]		0.5 mM

Sample #3

Temperature 303 (±2) K, pH 6.51 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	Methanobacterium thermoautotrophicum open reading frame 865	[U-10% 13C; U-100% 15N]		0.5 mM

nullKeywords helical bundle, heternuclear NMR, structural proteomics, Methanobacterium thermoautotrophicum

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Found 1 Document (1.605 seconds)

BMRB: 4996

Sample #1

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Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MTH865, : 1 : 4 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3