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BMRB: 50007

6UCJ

Backbone 1H, 13C, and 15N Chemical Shift Assignments for proIAPP in DPC Micelles

Authors

DeLisle, C.F., Malooley, A.L., Banerjee, I., Lorieau, J.L.

Assembly

proIAPP-K4D

Entity

1. proIAPP-K4D (polymer, Thiol state: all disulfide bound), 72 monomers, 8031.062 Da Detail

TPIESHQVEK RKCNTATCAT QRLANFLVHS SNNFGAILSS TNVGSNTYGK RNAVEVLKRE PLNYLPLKKK KD

Formula weight

8031.062 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 84.7 %, Completeness: 44.4 %, Completeness (bb): 71.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.4 % (375 of 844)	29.8 % (132 of 443)	57.5 % (185 of 322)	73.4 % (58 of 79)
Backbone	71.8 % (306 of 426)	56.9 % (82 of 144)	77.9 % (166 of 213)	84.1 % (58 of 69)
Sidechain	25.1 % (122 of 487)	16.7 % (50 of 299)	40.4 % (72 of 178)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 44)	0.0 % (0 of 22)	0.0 % (0 of 22)
Methyl	35.9 % (28 of 78)	33.3 % (13 of 39)	38.5 % (15 of 39)

1. entity 1

TPIESHQVEK RKCNTATCAT QRLANFLVHS SNNFGAILSS TNVGSNTYGK RNAVEVLKRE PLNYLPLKKK KD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 7.0, Details proIAPP-K4D_pH7.0

#	Name	Isotope labeling	Concentration
1	DPC	natural abundance	200 mM
2	TRIS	natural abundance	30 mM
3	sodium chloride	natural abundance	50 mM
4	D2O	[U-100% 2H]	10 v/v
5	sodium azide	natural abundance	0.03 %
6	proIAPP-K4D	[U-100% 13C; U-100% 15N]	1.0 (±0.2) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 7.0, Details proIAPP_pH4.5

#	Name	Isotope labeling	Concentration
7	DPC	natural abundance	200 mM
8	sodium acetate	natural abundance	30 mM
9	sodium chloride	natural abundance	50 mM
10	D2O	[U-100% 2H]	10 v/v
11	sodium azide	natural abundance	0.03 %
12	proIAPP-K4D	[U-100% 13C; U-100% 15N]	1.0 (±0.2) mM

Chem. Shift Complete2

Sequence coverage: 98.6 %, Completeness: 66.9 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.9 % (1130 of 1688)	59.1 % (524 of 886)	74.5 % (480 of 644)	79.7 % (126 of 158)
Backbone	84.6 % (721 of 852)	76.7 % (221 of 288)	88.0 % (375 of 426)	90.6 % (125 of 138)
Sidechain	54.4 % (530 of 974)	50.7 % (303 of 598)	63.5 % (226 of 356)	5.0 % (1 of 20)
Aromatic	12.5 % (11 of 88)	25.0 % (11 of 44)	0.0 % (0 of 44)
Methyl	66.7 % (104 of 156)	65.4 % (51 of 78)	67.9 % (53 of 78)

1. entity 1

TPIESHQVEK RKCNTATCAT QRLANFLVHS SNNFGAILSS TNVGSNTYGK RNAVEVLKRE PLNYLPLKKK KD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 4.5, Details proIAPP-K4D_pH7.0

#	Name	Isotope labeling	Concentration
1	DPC	natural abundance	200 mM
2	TRIS	natural abundance	30 mM
3	sodium chloride	natural abundance	50 mM
4	D2O	[U-100% 2H]	10 v/v
5	sodium azide	natural abundance	0.03 %
6	proIAPP-K4D	[U-100% 13C; U-100% 15N]	1.0 (±0.2) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 4.5, Details proIAPP_pH4.5

#	Name	Isotope labeling	Concentration
7	DPC	natural abundance	200 mM
8	sodium acetate	natural abundance	30 mM
9	sodium chloride	natural abundance	50 mM
10	D2O	[U-100% 2H]	10 v/v
11	sodium azide	natural abundance	0.03 %
12	proIAPP-K4D	[U-100% 13C; U-100% 15N]	1.0 (±0.2) mM

Release date

2019-09-02

Citation

Pro-islet amyloid polypeptide in micelles contains a helical prohormone segment
DeLisle, C.F., Malooley, A.L., Banerjee, I., Lorieau, J.L.
FEBS J. (2020), 287, 4440-4457, PubMed 32077246 , DOI 10.1111/febs.15253 , Abstract

Related entities 1. proIAPP-K4D, : 1 : 1 : 2 : 20 entities Detail

Interaction partners 1. proIAPP-K4D, : 2 interactors Detail

More details for 2 interactors identified by 2 citations