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BMRB: 5007

1J6Q

SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP

Authors

Flinders, J.C., Dieckmann, T.

Assembly

VS RIBOZYME SUBSTRATE STEM-LOOP

Entity

1. VS RIBOZYME SUBSTRATE STEM-LOOP (polymer, Thiol state: not present), 30 monomers, 9768.803 Da Detail

GGUGCGAAGG GCGUCGUCGC CCCGAGCGCC

Formula weight

9768.803 Da

Source organism

Neurospora crassa

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 62.6 %, Completeness (bb): 55.8 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	62.6 % (299 of 478)	66.4 % (176 of 265)	54.3 % (107 of 197)	100.0 % (16 of 16)
Suger, PO4	55.8 % (184 of 330)	56.1 % (101 of 180)	55.3 % (83 of 150)
Nucleobase	77.7 % (115 of 148)	88.2 % (75 of 85)	51.1 % (24 of 47)	100.0 % (16 of 16)
Aromatic	80.2 % (101 of 126)	96.8 % (61 of 63)	51.1 % (24 of 47)	100.0 % (16 of 16)

1. VS Ribozyme

GGUGCGAAGG GCGUCGUCGC CCCGAGCGCC

Show sample condition

Sample

Pressure 1 atm, Temperature 274 (±0.2) K, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Release date

2001-06-06

Citation

A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA
Flinders, J.C., Dieckmann, T.
J. Mol. Biol. (2001), 308, 665-679, PubMed 11350168 , DOI 10.1006/jmbi.2001.4627 , Abstract

Related entities 1. VS RIBOZYME SUBSTRATE STEM-LOOP, : 1 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-1H NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Sample #2

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	VS Ribozyme	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
3	VS Ribozyme	[98%-13C; 98%-15N]-Ade		1.0 mM

2 2D 1H-13C HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Sample #2

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	VS Ribozyme	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
3	VS Ribozyme	[98%-13C; 98%-15N]-Ade		1.0 mM

3 2D 1H-13C CT-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Sample #2

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	VS Ribozyme	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
3	VS Ribozyme	[98%-13C; 98%-15N]-Ade		1.0 mM

4 2D 1H-15N HMQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Sample #2

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	VS Ribozyme	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
3	VS Ribozyme	[98%-13C; 98%-15N]-Ade		1.0 mM

5 2D 1H-1H DQF-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Sample #2

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	VS Ribozyme	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
3	VS Ribozyme	[98%-13C; 98%-15N]-Ade		1.0 mM

6 3D 13C-1H-1H NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Sample #2

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	VS Ribozyme	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
3	VS Ribozyme	[98%-13C; 98%-15N]-Ade		1.0 mM

7 3D 1H-13C-1H HCCH-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	VS Ribozyme			1.5 mM

Sample #2

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
2	VS Ribozyme	[U-98% 13C; U-98% 15N]		1.0 mM

Sample #3

Pressure 1 atm, pH 6.0 (±0.3)

#	Name	Isotope labeling	Type	Concentration
3	VS Ribozyme	[98%-13C; 98%-15N]-Ade		1.0 mM

nullKeywords A+C BASE PAIR, PROTONATED ADENINE, STEM-LOOP, TANDEM GA, VS RIBOZYME