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BMRB: 5010

1JAJ

1H, 15N, and 13C chemical shift assignments for DNA polymerase X

Authors

Maciejewski, M.W., Pan, B., Shin, R., Denninger, A., Mullen, G.P.

Assembly

DNA Polymerase X

Entity

1. DNA Polymerase X (polymer, Thiol state: all free), 174 monomers, 20314.16 Da Detail

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

Formula weight

20314.16 Da

Source organism

African swine fever virus BA71V

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 85.8 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.8 % (1921 of 2238)	85.7 % (1010 of 1179)	84.2 % (738 of 877)	95.1 % (173 of 182)
Backbone	95.0 % (980 of 1032)	96.6 % (338 of 350)	93.6 % (481 of 514)	95.8 % (161 of 168)
Sidechain	79.7 % (1094 of 1372)	81.1 % (672 of 829)	77.5 % (410 of 529)	85.7 % (12 of 14)
Aromatic	47.5 % (76 of 160)	90.0 % (72 of 80)	3.8 % (3 of 79)	100.0 % (1 of 1)
Methyl	89.0 % (219 of 246)	90.2 % (111 of 123)	87.8 % (108 of 123)

1. DNA Polymerase X

MLTLIQGKKI VNHLRSRLAF EYNGQLIKIL SKNIVAVGSL RREEKMLNDV DLLIIVPEKK LLKHVLPNIR IKGLSFSVKV CGERKCVLFI EWEKKTYQLD LFTALAEEKP YAIFHFTGPV SYLIRIRAAL KKKNYKLNQY GLFKNQTLVP LKITTEKELI KELGFTYRIP KKRL

Show sample condition

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1JAJ, Strand ID: A Detail

Release date

2002-04-04

Citation 1

Letter to the Editor: 1H, 15N, and 13C Resonance Assignments for a 20 kDa DNA Polymerase from African Swine Fever Virus
Maciejewski, M.W., Pan, B., Shin, R., Denninger, A., Mullen, G.P.
J. Biomol. NMR (2001), 21, 177-178

Show more 3 citaions

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Citation 3

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
Bartels, C., Xia, T., Billeter, M., Guntert, P., Wuthrich, K.
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Citation 4

Analysis of the complete nucleotide sequence of African swine fever virus
Yanez, R.J., Rodriguez, J.M., Nogal, M.L., Yuste, L., Enriquez, C., Rodriguez, J.F., Vinuela, E.
Virology (1995), 208, 249-278, PubMed 11831707 , DOI 10.1006/viro.1995.1149 , Abstract

Hide non-primary 3 citaions

Related entities 1. DNA Polymerase X, : 1 : 20 : 2 : 3 : 104 entities Detail

Experiments performed 17 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

2 15N-edited NOESY-HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

3 HNCA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

4 HN(CO)CA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

5 HNCACB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

6 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

7 HNCO

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

8 HNHA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

9 HNHB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

10 HCC(CO)NH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

11 CC(CO)NH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

12 HCCH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

13 1H-13C HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

14 13C-edited NOESY-HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

15 13C-edited HSQC-NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

16 2D TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

17 2D NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
1	DNA Polymerase X	[U-13C; U-15N]	0.6 ~ 1.0 mM
2	PIPES		20 mM
3	NaCl		500 mM
4	DTT		10 mM
5	AEBSF		0.5 mM
6	sodium azide		0.02 %

Sample #2

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Isotope labeling	Concentration
7	DNA Polymerase X	[U-15N]	0.6 ~ 1.0 mM
8	PIPES		20 mM
9	NaCl		500 mM
10	DTT		10 mM
11	AEBSF		0.5 mM
12	sodium azide		0.02 %

Sample #3

Temperature 298 (±0.2) K, pH 6.5 (±0.25)

#	Name	Concentration
13	DNA Polymerase X	0.6 ~ 1.0 mM
14	PIPES	20 mM
15	NaCl	500 mM
16	DTT	10 mM
17	AEBSF	0.5 mM
18	sodium azide	0.02 %

nullKeywords DNA repair, African swine fever virus, polymerase

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BMRB: 5010

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Related entities 1. DNA Polymerase X, : 1 : 20 : 2 : 3 : 104 entities Detail

Experiments performed 17 experiments Detail

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