BMRBj - BMREntryMaster

Found 1 Document (1.804 seconds)

BMRB: 50117

UN2A

Authors

Zhou, T.

Assembly

UN2A assembly

Entity

1. UN2A assembly (polymer, Thiol state: not present), 111 monomers, 13323.90 Da Detail

MDERKKQEKI EGDLRAMLKK TPILKKGAGE EEEIDIMELL KNVDPKEYEK YARMYGITDF RGLLQAFELL KQSQEEETHR LEIEEIERSE RDEKEFEELV SFIQQRLSQT E

Formula weight

13323.9 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.8 %, Completeness: 31.7 %, Completeness (bb): 63.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	31.7 % (440 of 1389)	13.8 % (102 of 739)	49.7 % (261 of 525)	61.6 % (77 of 125)
Backbone	63.3 % (419 of 662)	38.2 % (86 of 225)	78.0 % (256 of 328)	70.6 % (77 of 109)
Sidechain	13.0 % (108 of 833)	3.1 % (16 of 514)	30.4 % (92 of 303)	0.0 % (0 of 16)
Aromatic	0.0 % (0 of 68)	0.0 % (0 of 34)	0.0 % (0 of 34)
Methyl	8.7 % (9 of 104)	5.8 % (3 of 52)	11.5 % (6 of 52)

1. entity 1

MDERKKQEKI EGDLRAMLKK TPILKKGAGE EEEIDIMELL KNVDPKEYEK YARMYGITDF RGLLQAFELL KQSQEEETHR LEIEEIERSE RDEKEFEELV SFIQQRLSQT E

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	UN2A	[U-13C; U-15N; U-2H]	1.2 mM
2	sodium phosphate	natural abundance	30 mM
3	sodium chloride	natural abundance	100 mM

Release date

2019-12-08

Citation

Molecular Characterisation of Titin N2A and Its Binding of CARP Reveals a Titin/Actin Cross-linking Mechanism
Zhou, T., Fleming, J.R., Lange, S., Hessel, A.L., Bogomolovas, J., Stronczek, C., Grundei, D., Ghassemian, M., Biju, A., Borgeson, E., Bullard, B., Linke, W.A., Chen, J., Kovermann, M., Mayans, O.
J. Mol. Biol. (2021), 433, 166901-166901, PubMed 33647290 , DOI 10.1016/j.jmb.2021.166901 , Abstract

Related entities 1. UN2A assembly, : 1 : 2 entities Detail

Interaction partners 1. UN2A assembly, : 53 interactors Detail

More details for 53 interactors identified by 24 citations

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50117_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50117_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	403		82.9 (chain: 1, length: 111)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 111, Sequence coverage: 82.9%
- Total number of assignments
  - ¹³C chemical shifts: 253
  - ¹H chemical shifts: 75
  - ¹⁵N chemical shifts: 75
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	222	167	75.2
¹H chemical shifts	225	75	33.3
¹⁵N chemical shifts	109	75	68.8

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Found 1 Document (1.804 seconds)

BMRB: 50117

Sample

Related entities 1. UN2A assembly, : 1 : 2 entities Detail

Interaction partners 1. UN2A assembly, : 53 interactors Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	525	253	48.2
¹H chemical shifts	739	75	10.1
¹⁵N chemical shifts	125	75	60.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	303	86	28.4
¹H chemical shifts	514	0	0.0
¹⁵N chemical shifts	16	0	0.0