BMRBj - BMREntryMaster

Found 1 Document (2.093 seconds)

BMRB: 5012

1J7M

Gelatin-binding Region of Human Matrix Metalloproteinase-2: Solution Structure, Dynamics, and Function of the COL-23 Two-domain Construct

Authors

Briknarova, K., Gehrmann, M., Banyai, L., Tordai, H., Patthy, L., Llinas, M.

Assembly

the third domain of MMP-2

Entity

1. the third domain of MMP-2 (polymer, Thiol state: all disulfide bound), 72 monomers, 7745.505 Da Detail

LAAHPPFASW MSTVGGNSGG APCVFPFTFL GNKYESCTSA GRSDGKMWCA TTANYDDDRK WGFCPDQGWI SS

Formula weight

7745.505 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS23:SG	1:CYS49:SG
2	disulfide	sing	1:CYS37:SG	1:CYS64:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 93.3 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	93.3 % (446 of 478)	92.6 % (373 of 403)	97.3 % (73 of 75)
Backbone	98.1 % (211 of 215)	98.0 % (145 of 148)	98.5 % (66 of 67)
Sidechain	89.4 % (235 of 263)	89.4 % (228 of 255)	87.5 % (7 of 8)
Aromatic	92.1 % (58 of 63)	91.5 % (54 of 59)	100.0 % (4 of 4)
Methyl	100.0 % (22 of 22)	100.0 % (22 of 22)

1. Matrix Metalloproteinase 2

LAAHPPFASW MSTVGGNSGG APCVFPFTFL GNKYESCTSA GRSDGKMWCA TTANYDDDRK WGFCPDQGWI SS

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

Protein Blocks Logo

Calculated from 50 models in PDB: 1J7M, Strand ID: A Detail

Release date

2001-05-17

Citation

Gelatin-binding region of human matrix metalloproteinase-2: solution structure, dynamics, and function of the COL-23 two-domain construct
Briknarova, K., Gehrmann, M., Banyai, L., Tordai, H., Patthy, L., Llinas, M.
J. Biol. Chem. (2001), 276, 27613-27621, PubMed 11320090 , DOI 10.1074/jbc.M101105200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P08253 released on 1988-08-01
Title MMP2_HUMAN Entity 72 kDa type IV collagenase

Related entities 1. the third domain of MMP-2, : 1 : 62 entities Detail

Experiments performed 7 experiments Detail

1 2D COSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

2 2D TOCSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

3 2D NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

4 3D 15N NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

5 3D 15N TOCSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

6 HNHB

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

7 HSQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Matrix Metalloproteinase 2			0.5 mM

Sample #2

Temperature 298 (±0.5) K, pH 5.4 (±0.02)

#	Name	Isotope labeling	Type	Concentration
2	Matrix Metalloproteinase 2	[U-15N]		1 mM

nullKeywords col domains, fibronectin type II modules, gelatinase A, matrix metalloproteinase 2, type IV collagenase

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.093 seconds)

BMRB: 5012

Sample #1

Sample #2

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. the third domain of MMP-2, : 1 : 62 entities Detail

Experiments performed 7 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2