DnaJB1 J-domain+G/F rich region
GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.6 % (964 of 1243) | 69.2 % (450 of 650) | 88.2 % (419 of 475) | 80.5 % (95 of 118) |
Backbone | 87.1 % (561 of 644) | 77.0 % (177 of 230) | 93.2 % (289 of 310) | 91.3 % (95 of 104) |
Sidechain | 71.1 % (491 of 691) | 65.0 % (273 of 420) | 84.8 % (218 of 257) | 0.0 % (0 of 14) |
Aromatic | 86.2 % (119 of 138) | 82.6 % (57 of 69) | 89.9 % (62 of 69) | |
Methyl | 100.0 % (62 of 62) | 100.0 % (31 of 31) | 100.0 % (31 of 31) |
1. B1GF
GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRNSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DnaJB1GF | [U-15N; U-13C] | 4 mM | |
2 | HEPES | natural abundance | 50 mM | |
3 | potassium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.03 % | |
5 | D2O | [U-2H] | 10 % |
Solvent system 99.9% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | DnaJB1GF | [U-15N;U-13C] | 4 mM | |
7 | HEPES | natural abundance | 50 mM | |
8 | potassium chloride | natural abundance | 50 mM | |
9 | sodium azide | natural abundance | 0.03 % | |
10 | D2O | [U-2H] | 99.9 % |