BMRB: 5018

Solution Structure of the Tumor Necrosis Factor Receptor-1 Death Domain

Authors

Sukits, S.F., Lin, L., Hsu, S., Malakian, K., Powers, R., Xu, G.

Assembly

TUMOR NECROSIS FACTOR RECEPTOR-1

Entity

1. TUMOR NECROSIS FACTOR RECEPTOR-1 (polymer, Thiol state: all free), 112 monomers, 12789.63 Da Detail

MAHKPQSLDT DDPATLYAVV ENVPPLRWKE FVKRLGLSDH EIDRLELQNG RCLREAQYSM LATWRRRTPR REATLELLGR VLRDMDLLGC LEDIEEALCG PAALPPAPSL LR

Formula weight

12789.63 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Distance geometry, Simulated annealing molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 77.9 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.9 % (1020 of 1309)	70.8 % (487 of 688)	84.9 % (434 of 511)	90.0 % (99 of 110)
Backbone	91.0 % (595 of 654)	90.9 % (200 of 220)	90.3 % (299 of 331)	93.2 % (96 of 103)
Sidechain	68.9 % (525 of 762)	61.3 % (287 of 468)	81.9 % (235 of 287)	42.9 % (3 of 7)
Aromatic	62.1 % (36 of 58)	62.1 % (18 of 29)	59.3 % (16 of 27)	100.0 % (2 of 2)
Methyl	76.8 % (106 of 138)	73.9 % (51 of 69)	79.7 % (55 of 69)

1. Tumor Necrosis Factor Receptor-1

MAHKPQSLDT DDPATLYAVV ENVPPLRWKE FVKRLGLSDH EIDRLELQNG RCLREAQYSM LATWRRRTPR REATLELLGR VLRDMDLLGC LEDIEEALCG PAALPPAPSL LR

Show sample condition

Sample

Pressure 1 atm, Temperature 308 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
1	Tumor Necrosis Factor Receptor-1			1 mM
2	sodium acetate			10 mM
3	DTT			10 mM

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0
13C	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 1 models in PDB: 1ICH, Strand ID: A Detail

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Release date

2002-05-08

Citation

Solution structure of the tumor necrosis factor receptor-1 death domain
Sukits, S.F., Lin, L., Hsu, S., Malakian, K., Powers, R., Xu, G.
J. Mol. Biol. (2001), 310, 895-906, PubMed 11453696 , DOI 10.1006/jmbi.2001.4790 , Abstract

Related entities 1. TUMOR NECROSIS FACTOR RECEPTOR-1, : 1 : 10 entities Detail

Interaction partners 1. TUMOR NECROSIS FACTOR RECEPTOR-1, : 102 interactors Detail

Experiments performed 3 experiments Detail