BMRBj - BMREntryMaster

Found 1 Document (0.636 seconds)

BMRB: 50219

6X43

Mfd_RID

Authors

Kawale, A.A., Burmann, B.M.

Assembly

RID

Entity

1. RID (polymer, Thiol state: not present), 76 monomers, 8100.122 Da Detail

RNLAELHIGQ PVVHLEHGVG RYAGMTTLEA GGITGEYLML TYANDAKLYV PVSSLHLISR YAGGAEENAP LHKLGG

Formula weight

8100.122 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 90.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.2 % (756 of 838)	91.1 % (388 of 426)	91.9 % (305 of 332)	78.8 % (63 of 80)
Backbone	90.7 % (408 of 450)	91.3 % (146 of 160)	92.2 % (200 of 217)	84.9 % (62 of 73)
Sidechain	89.2 % (404 of 453)	89.5 % (238 of 266)	92.2 % (166 of 180)	0.0 % (0 of 7)
Aromatic	66.7 % (40 of 60)	66.7 % (20 of 30)	66.7 % (20 of 30)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. entity 1

RNLAELHIGQ PVVHLEHGVG RYAGMTTLEA GGITGEYLML TYANDAKLYV PVSSLHLISR YAGGAEENAP LHKLGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6YHZ, Strand ID: A Detail

Release date

2020-03-29

Citation

Publisher Correction: UvrD helicase-RNA polymerase interactions are governed by UvrD's carboxy-terminal Tudor domain
Kawale, A.A., Burmann, B.M.
Commun. Biol. (2020), 3, 661-661, PubMed 33154434 , DOI 10.1038/s42003-020-01426-x , Abstract

Related entities 1. RID, : 1 : 11 : 40 entities Detail

Interaction partners 1. RID, : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

5 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

6 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

8 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

9 3D HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

10 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

12 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

13 2D HBCBCGCDCEHE

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

14 2D HBCBCGCDHD

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details [NaCl]: 137 mM [KCl]: 2.7 mM [Na2HPO4]: 10 mM [KH2PO4]: 1.8 mM

#	Name	Isotope labeling	Type	Concentration
1	RID	[U-100% 13C; U-100% 15N]		600 uM
2	1x PBS buffer	natural abundance		152 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50219_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr50219_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	854		100.0 (chain: 1, length: 76)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 76, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 488
  - ¹³C chemical shifts: 303
  - ¹⁵N chemical shifts: 63
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords UvrD, Mfd, Tudor, TCR, DNA repair