BMRBj - BMREntryMaster

Found 1 Document (1.875 seconds)

BMRB: 5022

1I4V

Converting a DNA Damage Checkpoint Effector (UmuD2C) into a Lesion Bypass Polymerase (UmuD'2C)

Authors

Ferentz, A.E., Walker, G.C., Wagner, G.

Assembly

UMUD PROTEIN

Entity

1. UMUD PROTEIN (polymer, Thiol state: not present), 115 monomers, 12298.90 × 2 Da Detail

AFPSPAADYV EQRIDLNQLL IQHPSATYFV KASGDSMIDG GISDGDLLIV DSAITASHGD IVIAAVDGEF TVKKLQLRPT VQLIPMNSAY SPITISSEDT LDVFGVVIHV VKAMR

Total weight

24597.8 Da

Max. entity weight

12298.9 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 68.8 %, Completeness (bb): 79.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.8 % (880 of 1280)	72.2 % (470 of 651)	59.1 % (303 of 513)	92.2 % (107 of 116)
Backbone	79.9 % (542 of 678)	96.1 % (222 of 231)	63.0 % (213 of 338)	98.2 % (107 of 109)
Sidechain	61.3 % (435 of 710)	59.0 % (248 of 420)	66.1 % (187 of 283)	0.0 % (0 of 7)
Aromatic	21.1 % (16 of 76)	42.1 % (16 of 38)	0.0 % (0 of 38)
Methyl	81.9 % (136 of 166)	84.3 % (70 of 83)	79.5 % (66 of 83)

1. UMUD PROTEIN

AFPSPAADYV EQRIDLNQLL IQHPSATYFV KASGDSMIDG GISDGDLLIV DSAITASHGD IVIAAVDGEF TVKKLQLRPT VQLIPMNSAY SPITISSEDT LDVFGVVIHV VKAMR

Show sample condition

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	UMUD PROTEIN	[U-15N]	0.9 mM
2	NaCl		150 mM
3	phosphate		10 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	H2O		95 %
7	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	UMUD PROTEIN	[U-15N; U-13C]	1.3 mM
9	NaCl		150 mM
10	phosphate		20 mM
11	DTT		1 mM
12	EDTA		0.1 mM
13	H2O		95 %
14	D2O		5 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1I4V, Strand ID: A, B Detail

Release date

2003-01-06

Citation

Converting a DNA damage checkpoint effector (UmuD2C) into a lesion bypass polymerase (UmuD'2C)
Ferentz, A.E., Walker, G.C., Wagner, G.
EMBO J. (2001), 20, 4287-4298, PubMed 11483531 , DOI 10.1093/emboj/20.15.4287 , Abstract

Related entities 1. UMUD PROTEIN, : 1 : 5 : 79 entities Detail

Experiments performed 4 experiments Detail

1 HNHA

Instrument

Model Varian VXR (500 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	UMUD PROTEIN	[U-15N]	0.9 mM
2	NaCl		150 mM
3	phosphate		10 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	H2O		95 %
7	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	UMUD PROTEIN	[U-15N; U-13C]	1.3 mM
9	NaCl		150 mM
10	phosphate		20 mM
11	DTT		1 mM
12	EDTA		0.1 mM
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
15	UMUD PROTEIN	1.5 mM
16	NaCl	150 mM
17	phosphate	10 mM
18	DTT	1 mM
19	EDTA	0.1 mM
20	H2O	95 %
21	D2O	5 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
22	UMUD PROTEIN	1.4 mM
23	NaCl	150 mM
24	phosphate	10 mM
25	DTT	1 mM
26	EDTA	0.1 mM
27	D2O	100 %

2 2D NOESY

Instrument

Model Varian VXR (500 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	UMUD PROTEIN	[U-15N]	0.9 mM
2	NaCl		150 mM
3	phosphate		10 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	H2O		95 %
7	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	UMUD PROTEIN	[U-15N; U-13C]	1.3 mM
9	NaCl		150 mM
10	phosphate		20 mM
11	DTT		1 mM
12	EDTA		0.1 mM
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
15	UMUD PROTEIN	1.5 mM
16	NaCl	150 mM
17	phosphate	10 mM
18	DTT	1 mM
19	EDTA	0.1 mM
20	H2O	95 %
21	D2O	5 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
22	UMUD PROTEIN	1.4 mM
23	NaCl	150 mM
24	phosphate	10 mM
25	DTT	1 mM
26	EDTA	0.1 mM
27	D2O	100 %

3 3D 13C-separated NOESY

Instrument

Model Varian VXR (500 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	UMUD PROTEIN	[U-15N]	0.9 mM
2	NaCl		150 mM
3	phosphate		10 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	H2O		95 %
7	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	UMUD PROTEIN	[U-15N; U-13C]	1.3 mM
9	NaCl		150 mM
10	phosphate		20 mM
11	DTT		1 mM
12	EDTA		0.1 mM
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
15	UMUD PROTEIN	1.5 mM
16	NaCl	150 mM
17	phosphate	10 mM
18	DTT	1 mM
19	EDTA	0.1 mM
20	H2O	95 %
21	D2O	5 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
22	UMUD PROTEIN	1.4 mM
23	NaCl	150 mM
24	phosphate	10 mM
25	DTT	1 mM
26	EDTA	0.1 mM
27	D2O	100 %

4 3D 15N-separated NOESY

Instrument

Model Varian VXR (500 MHz)

Sample #1

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	UMUD PROTEIN	[U-15N]	0.9 mM
2	NaCl		150 mM
3	phosphate		10 mM
4	DTT		1 mM
5	EDTA		0.1 mM
6	H2O		95 %
7	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Concentration
8	UMUD PROTEIN	[U-15N; U-13C]	1.3 mM
9	NaCl		150 mM
10	phosphate		20 mM
11	DTT		1 mM
12	EDTA		0.1 mM
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
15	UMUD PROTEIN	1.5 mM
16	NaCl	150 mM
17	phosphate	10 mM
18	DTT	1 mM
19	EDTA	0.1 mM
20	H2O	95 %
21	D2O	5 %

Sample #4

Pressure 1 atm, Temperature 303 K, pH 6.0

#	Name	Concentration
22	UMUD PROTEIN	1.4 mM
23	NaCl	150 mM
24	phosphate	10 mM
25	DTT	1 mM
26	EDTA	0.1 mM
27	D2O	100 %

nullKeywords SOS response, SOS mutagenesis, DNA repair, DNA polymerase V, DNA polymerase accessory protein, LexA repressor, lambda CI, signal peptidase, serine-lysine dyad, autocatalytic cleavage, serine protease

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Found 1 Document (1.875 seconds)

BMRB: 5022

Sample #1

Sample #2

Related entities 1. UMUD PROTEIN, : 1 : 5 : 79 entities Detail

Experiments performed 4 experiments Detail

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Sample #2

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Sample #4

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