BMRB: 5023

Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter

Authors

Tinoco, I., Kim, C., Kao, C.C.

Assembly

5'-R(*GP*GP*UP*GP*CP*UP*UP*AP*GP*CP*AP*CP*C)-3'

Entity

1. 5'-R(*GP*GP*UP*GP*CP*UP*UP*AP*GP*CP*AP*CP*C)-3' (polymer, Thiol state: not present), 13 monomers, 4196.474 Da Detail

GGUGCUUAGC ACC

Formula weight

4196.474 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 38.5 %, Completeness: 8.7 %, Completeness (bb): 12.8 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	8.7 % (10 of 115)	8.7 % (10 of 115)
Suger, PO4	12.8 % (10 of 78)	12.8 % (10 of 78)
Nucleobase	0.0 % (0 of 37)	0.0 % (0 of 37)
Aromatic	0.0 % (0 of 29)	0.0 % (0 of 29)

1. mutant BMV (+) strand RNA

GGUGCUUAGC ACC

Show sample condition

Sample

Pressure 1 atm, Temperature 283 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	mutant BMV (+) strand RNA			2 mM
2	sodium phosphate buffer			5 mM
3	H2O			90 %
4	D2O			10 %

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Release date

2001-08-19

Citation

Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter
Tinoco, I., Kim, C.
J. Mol. Biol. (2001), 307, 827-839, PubMed 11273704 , DOI 10.1006/jmbi.2001.4497 , Abstract

Related entities 1. 5'-R(*GP*GP*UP*GP*CP*UP*UP*AP*GP*CP*AP*CP*C)-3', : 1 : 1 entities Detail

Experiments performed 2 experiments Detail