BMRB: 5024

The NMR solution structure of alpha-bungarotoxin

Authors

Zeng, H., Moise, L., Grant, M.A., Hawrot, E.

Assembly

ALPHA-BUNGAROTOXIN

Entity

1. ALPHA-BUNGAROTOXIN (polymer, Thiol state: all disulfide bound), 74 monomers, 7994.189 Da Detail

IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG

Formula weight

7994.189 Da

Entity Connection

disulfide 5 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS23:SG
2	disulfide	sing	1:CYS16:SG	1:CYS44:SG
3	disulfide	sing	1:CYS29:SG	1:CYS33:SG
4	disulfide	sing	1:CYS48:SG	1:CYS59:SG
5	disulfide	sing	1:CYS60:SG	1:CYS65:SG

Source organism

Bungarus multicinctus

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 62.0 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	62.0 % (261 of 421)	62.0 % (261 of 421)
Backbone	95.8 % (138 of 144)	95.8 % (138 of 144)
Sidechain	44.4 % (123 of 277)	44.4 % (123 of 277)
Aromatic	13.0 % (3 of 23)	13.0 % (3 of 23)
Methyl	63.3 % (19 of 30)	63.3 % (19 of 30)

1. alpha-neurotoxin

IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG

Show sample condition

Sample

Pressure 1 atm, Temperature 308 (±1) K, pH 4.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	alpha-neurotoxin			2.0 mM
2	potassium acetate	[U-2H]		50 mM
3	H2O			95 %
4	D2O			5 %
5	sodium azide			0.05 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 1IDL, Strand ID: A Detail

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Release date

2001-05-20

Citation

The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica
Zeng, H., Moise, L., Grant, M.A., Hawrot, E.
J. Biol. Chem. (2001), 276, 22930-22940, PubMed 11312275 , DOI 10.1074/jbc.M102300200 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. ALPHA-BUNGAROTOXIN, : 1 : 22 : 9 : 124 entities Detail

Interaction partners 1. ALPHA-BUNGAROTOXIN, : 5 interactors Detail

Experiments performed 2 experiments Detail