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Found 1 Document (1.664 seconds)

BMRB: 5030

1TUJ

1H, 13C and 15N Chemical Shift Assignment of the Honeybee Odorant-binding Protein ASP2

Authors

Lescop, E., Briand, L., Pernollet, J., van Heijenoort, C., Guittet, E.

Assembly

Honeybee antennal specific Protein 2

Entity

1. Honeybee antennal specific Protein 2 (polymer, Thiol state: all disulfide bound), 123 monomers, 13700.63 Da Detail

IDQDTVVAKY MEYLMPDIMP CADELHISED IATNIQAAKN GADMSQLGCL KACVMKRIEM LKGTELYVEP VYKMIEVVHA GNADDIQLVK GIANECIENA KGETDECNIG NKYTDCYIEK LFS

Formula weight

13700.63 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS21:SG	1:CYS53:SG
2	disulfide	sing	1:CYS49:SG	1:CYS107:SG
3	disulfide	sing	1:CYS96:SG	1:CYS116:SG

Source organism

Apis mellifera

Exptl. method

NMR

Refine. method

automatic NOE assignment using ARIA combined with CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 69.9 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.9 % (978 of 1399)	65.6 % (475 of 724)	70.4 % (383 of 544)	91.6 % (120 of 131)
Backbone	100.0 % (732 of 732)	100.0 % (250 of 250)	100.0 % (362 of 362)	100.0 % (120 of 120)
Sidechain	46.2 % (362 of 783)	47.5 % (225 of 474)	46.0 % (137 of 298)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 66)	0.0 % (0 of 33)	0.0 % (0 of 33)
Methyl	30.6 % (44 of 144)	29.2 % (21 of 72)	31.9 % (23 of 72)

1. ASP2

IDQDTVVAKY MEYLMPDIMP CADELHISED IATNIQAAKN GADMSQLGCL KACVMKRIEM LKGTELYVEP VYKMIEVVHA GNADDIQLVK GIANECIENA KGETDECNIG NKYTDCYIEK LFS

Show sample condition

Sample #1

Temperature 308 (±1) K, pH 7.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K, pH 7.7 (±0.1)

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K, pH 7.7 (±0.1)

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

Protein Blocks Logo

Calculated from 17 models in PDB: 1TUJ, Strand ID: A Detail

Release date

2001-05-22

Citation 1

1H, 13C and 15N chemical shift assignment of the honeybee odorant-binding protein ASP2
Lescop, E., Briand, L., Pernollet, J., van Heijenoort, C., Guittet, E.
J. Biomol. NMR (2001), 21, 181-182, PubMed 11727984 , Abstract

Show more 3 citaions

Citation 2

Ligand binding and physico-chemical properties of ASP2, a recombinant odorant-binding protein from honeybee (Apis mellifera L.)
Briand, L., Nespoulous, C., Huet, J.C., Takahashi, M., Pernollet, J.C.
Eur. J. Biochem. (2001), 268, 752-760, PubMed 11168415 , DOI: , Abstract

Citation 3

Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris
Briand, L., Perez, V., Huet, J.C., Danty, E., Masson, C., Pernollet, J.C.
Protein Expr. Purif. (1999), 15, 362-369, PubMed 10092496 , DOI: , Abstract

Citation 4

Cloning and expression of a queen pheromone-binding protein in the honeybee: an olfactory-specific, developmentally regulated protein
Danty, E., Briand, L., Michard-Vanhee, C., Perez, V., Arnold, G., Gaudemer, O., Huet, D., Huet, J.C., Ouali, C., Masson, C., Pernollet, J.C.
J. Neurosci. (1999), 19, 7468-7475, PubMed 10460253 , Abstract

Hide non-primary 3 citaions

Related entities 1. Honeybee antennal specific Protein 2, : 1 : 3 entities Detail

Experiments performed 10 experiments Detail

1 1H-15N NOESY

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

2 1H-15N TOCSY

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

3 HNCA

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

4 HN(CO)CA

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

5 HNCO

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

6 HN(CA)CO

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

7 HNCACB

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

8 CBCA(CO)NH

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

9 HCCH-TOCSY

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

10 NOESY-HSQC

Instrument

Model Bruker DRX (800 MHz) Details Spectrometer equipped with a 5 mm TXI triple resonance, triple-axis gradient probe.

Sample #1

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
1	ASP2	[U-98% 13C; U-98% 15N]	0.7 ~ 1.5 mM
2	TSP		0.1 mM
3	phosphate buffer		100 mM
4	NaN3		0.1 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
7	ASP2	[U-98% 13C; U-98% 15N]	1.3 mM
8	TSP		0.1 mM
9	phosphate buffer		100 mM
10	NaN3		0.1 mM
11	D2O		100 %

Sample #3

Temperature 308 (±1) K

#	Name	Isotope labeling	Concentration
12	ASP2	[U-98% 15N]	1 mM
13	TSP		0.1 mM
14	phosphate buffer		100 mM
15	NaN3		0.1 mM
16	H2O		90 %
17	D2O		10 %

nullKeywords apis mellifera L., insect, odorant binding protein, olfaction, resonances assignments

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Found 1 Document (1.664 seconds)

BMRB: 5030

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Related entities 1. Honeybee antennal specific Protein 2, : 1 : 3 entities Detail

Experiments performed 10 experiments Detail

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