BMRB: 5031

Sequence-specific 1H, 13C and 15N resonance assignments of the N-terminal, 135-residue domain of KaiA, a clock protein from Synechococcus elongatus

Authors

Vakonakis, I., Risinger, A.T., Latham, M.P., Williams, S.B., Golden, S.S., LiWang, A.C.

Assembly

KaiA N-terminus

Entity

1. KaiA N-terminus (polymer, Thiol state: all free), 135 monomers, 15066.02 Da Detail

MLSQIAICIW VESTAILQDC QRALSADRYQ LQVCESGEML LEYAQTHRDQ IDCLILVAAN PSFRAVVQQL CFEGVVVPAI VVGDRDSEDP DEPAKEQLYH SAELHLGIHQ LEQLPYQVDA ALAEFLRLAP VETMA

Formula weight

15066.02 Da

Source organism

Synechococcus elongatus

Exptl. method

NMR

Refine. method

Distance geometry, Simulated annealing regularization, Simulated annealing refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 94.6 %, Completeness (bb): 95.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.6 % (1459 of 1542)	95.2 % (755 of 793)	94.0 % (569 of 605)	93.8 % (135 of 144)
Backbone	95.4 % (761 of 798)	95.5 % (256 of 268)	95.5 % (383 of 401)	94.6 % (122 of 129)
Sidechain	94.1 % (823 of 875)	95.0 % (499 of 525)	92.8 % (311 of 335)	86.7 % (13 of 15)
Aromatic	86.7 % (78 of 90)	88.9 % (40 of 45)	84.1 % (37 of 44)	100.0 % (1 of 1)
Methyl	97.3 % (181 of 186)	98.9 % (92 of 93)	95.7 % (89 of 93)

1. KaiA

MLSQIAICIW VESTAILQDC QRALSADRYQ LQVCESGEML LEYAQTHRDQ IDCLILVAAN PSFRAVVQQL CFEGVVVPAI VVGDRDSEDP DEPAKEQLYH SAELHLGIHQ LEQLPYQVDA ALAEFLRLAP VETMA

Show sample condition

Sample

Temperature 298 (±0.05) K, pH 6.4 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	KaiA	[U-99% 13C; U-99% 15N]		2.9 ~ 3.4 mM

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1M2E, Strand ID: A Detail

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Release date

2001-05-24

Citation

Sequence-specific 1H, 13C and 15N resonance assignments of the N-terminal, 135-residue domain of KaiA, a clock protein from Synechococcus elongatus
Vakonakis, I., Risinger, A.T., Latham, M.P., Williams, S.B., Golden, S.S., LiWang, A.C.
J. Biomol. NMR (2001), 21, 179-180, PubMed 11727983 , DOI: , Abstract

Related entities 1. KaiA N-terminus, : 1 : 4 : 2 : 5 entities Detail

Interaction partners 1. KaiA N-terminus, : 5 interactors Detail

Experiments performed 8 experiments Detail