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Found 1 Document (1.942 seconds)

BMRB: 5036

1I11

Solution Structure and Backbone Dynamics of the DNA-Binding Domain of Mouse Sox-5

Authors

Cary, P.D., Read, C.M., Davis, B., Driscoll, P.C., Crane-Robinson, C.

Assembly

TRANSCRIPTION FACTOR SOX-5

Entity

1. TRANSCRIPTION FACTOR SOX-5 (polymer, Thiol state: not present), 81 monomers, 9804.239 Da Detail

GSPHIKRPMN AFMVWAKDER RKILQAFPDM HNSNISKILG SRWKAMTNLE KQPYYEEQAR LSKQHLEKYP DYKYKPRPKR T

Formula weight

9804.239 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

SIMULATING ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.7 %, Completeness: 77.5 %, Completeness (bb): 87.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	77.5 % (500 of 645)	75.8 % (425 of 561)	89.3 % (75 of 84)
Backbone	87.9 % (203 of 231)	87.9 % (138 of 157)	87.8 % (65 of 74)
Sidechain	71.7 % (297 of 414)	71.0 % (287 of 404)	100.0 % (10 of 10)
Aromatic	68.0 % (34 of 50)	66.7 % (32 of 48)	100.0 % (2 of 2)
Methyl	96.3 % (26 of 27)	96.3 % (26 of 27)

1. TRANSCRIPTION FACTOR SOX-5

GSPHIKRPMN AFMVWAKDER RKILQAFPDM HNSNISKILG SRWKAMTNLE KQPYYEEQAR LSKQHLEKYP DYKYKPRPKR T

Show sample condition

Sample

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Isotope labeling	Concentration
1	TRANSCRIPTION FACTOR SOX-5	[U-15N]	2.0 ~ 3.0 mM
2	potassium phosphate buffer		75 mM
3	DTT		0.5 mM
4	H2O		90 %
5	D2O		10 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1I11, Strand ID: A Detail

Release date

2001-07-04

Citation

Solution structure and backbone dynamics of the DNA-binding domain of mouse Sox-5
Cary, P.D., Read, C.M., Davis, B., Driscoll, P.C., Crane-Robinson, C.
Protein Sci. (2001), 10, 83-98, PubMed 11266597 , DOI 10.1110/ps.32801 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P35710 released on 1994-06-01
Title SOX5_MOUSE Entity Transcription factor SOX-5

Related entities 1. TRANSCRIPTION FACTOR SOX-5, : 1 : 6 : 245 entities Detail

Interaction partners 1. TRANSCRIPTION FACTOR SOX-5, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 3 experiments Detail

1 2D 1H NOESY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Isotope labeling	Concentration
1	TRANSCRIPTION FACTOR SOX-5	[U-15N]	2.0 ~ 3.0 mM
2	potassium phosphate buffer		75 mM
3	DTT		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Concentration
6	TRANSCRIPTION FACTOR SOX-5	3 mM
7	potassium phosphate buffer	75 mM
8	DTT	0.5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Isotope labeling	Concentration
10	TRANSCRIPTION FACTOR SOX-5	[U-15N]	1 mM
11	potassium phosphate buffer		75 mM
12	DTT		0.5 mM
13	H2O		90 %
14	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Concentration
15	TRANSCRIPTION FACTOR SOX-5	3 mM
16	potassium phosphate buffer	75 mM
17	DTT	0.5 mM
18	H2O	90 %
19	D2O	10 %

2 2D 15N-1H HMQC-J

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Isotope labeling	Concentration
1	TRANSCRIPTION FACTOR SOX-5	[U-15N]	2.0 ~ 3.0 mM
2	potassium phosphate buffer		75 mM
3	DTT		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Concentration
6	TRANSCRIPTION FACTOR SOX-5	3 mM
7	potassium phosphate buffer	75 mM
8	DTT	0.5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Isotope labeling	Concentration
10	TRANSCRIPTION FACTOR SOX-5	[U-15N]	1 mM
11	potassium phosphate buffer		75 mM
12	DTT		0.5 mM
13	H2O		90 %
14	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Concentration
15	TRANSCRIPTION FACTOR SOX-5	3 mM
16	potassium phosphate buffer	75 mM
17	DTT	0.5 mM
18	H2O	90 %
19	D2O	10 %

3 3D 15N-1H NOESY

Instrument

Model Varian UNITYplus (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Isotope labeling	Concentration
1	TRANSCRIPTION FACTOR SOX-5	[U-15N]	2.0 ~ 3.0 mM
2	potassium phosphate buffer		75 mM
3	DTT		0.5 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Concentration
6	TRANSCRIPTION FACTOR SOX-5	3 mM
7	potassium phosphate buffer	75 mM
8	DTT	0.5 mM
9	D2O	100 %

Sample #3

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Isotope labeling	Concentration
10	TRANSCRIPTION FACTOR SOX-5	[U-15N]	1 mM
11	potassium phosphate buffer		75 mM
12	DTT		0.5 mM
13	H2O		90 %
14	D2O		10 %

Sample #4

Pressure 1 atm, Temperature 298.00 K, pH 6.20

#	Name	Concentration
15	TRANSCRIPTION FACTOR SOX-5	3 mM
16	potassium phosphate buffer	75 mM
17	DTT	0.5 mM
18	H2O	90 %
19	D2O	10 %

nullKeywords HMG box, DNA bending, DNA recognition, chromatin, NMR, DNA binding protein, DNA sequence specific, testis determining

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Found 1 Document (1.942 seconds)

BMRB: 5036

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. TRANSCRIPTION FACTOR SOX-5, : 1 : 6 : 245 entities Detail

Interaction partners 1. TRANSCRIPTION FACTOR SOX-5, : 1 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

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Sample #2

Sample #3

Sample #4

Instrument

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Sample #2

Sample #3

Sample #4