BMRB: 5038

Assignments for human ubiquitin-conjugating enzyme 2b (HsUbc2b)

Authors

Miura, T., Klaus, W., Ross, A., Guntert, P., Senn, H.

Assembly

HsUbc2b

Entity

1. HsUbc2b (polymer, Thiol state: all free), 152 monomers, 17312.14 Da Detail

MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

Formula weight

17312.14 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 85.4 %, Completeness (bb): 81.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.4 % (1534 of 1797)	93.9 % (887 of 945)	72.3 % (500 of 692)	91.9 % (147 of 160)
Backbone	81.3 % (719 of 884)	94.9 % (281 of 296)	68.7 % (309 of 450)	93.5 % (129 of 138)
Sidechain	90.3 % (956 of 1059)	93.4 % (606 of 649)	85.6 % (332 of 388)	81.8 % (18 of 22)
Aromatic	70.7 % (106 of 150)	92.0 % (69 of 75)	48.6 % (35 of 72)	66.7 % (2 of 3)
Methyl	99.3 % (135 of 136)	98.5 % (67 of 68)	100.0 % (68 of 68)

1. ubiquitin-conjugating enzyme 2b

MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN DS

Show sample condition

Sample

Temperature 308 (±0.01) K, pH 6.7 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-conjugating enzyme 2b	[U-13C; U-15N]		1.0 mM
2	ammonium acetate	[U-2H]		75 mM
3	ammonium sulfate			100 mM

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1JAS, Strand ID: A Detail

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Release date

2001-06-07

Citation 1

The NMR structure of the class I human ubiquitin-conjugating enzyme 2b
Miura, T., Klaus, W., Ross, A., Guntert, P., Senn, H.
J. Biomol. NMR (2002), 22, 89-92, PubMed 11885984 , Abstract

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Citation 2

Characterization of the binding interface between ubiquitin and class I human ubiquitin-conjugating enzyme 2b by multidimensional heteronuclear NMR spectroscopy in solution
Miura, T., Klaus, W., Gsell, B., Miyamoto, C., Senn, H.
J. Mol. Biol. (1999), 290, 213-228, PubMed 10388568 , DOI 10.1006/jmbi.1999.2859 , Abstract

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. HsUbc2b, : 1 : 7 : 289 entities Detail

Interaction partners 1. HsUbc2b, : 11 interactors Detail