BMRB: 5042

1H, 13C, and 15N Chemical Shift Assignments for Human Lymphotactin

Authors

Kuloglu, E.SONAY., McCaslin, D.R., Kitabwalla, M., Pauza, C.DAVID., Markley, J.L., Volkman, B.F.

Assembly

Human lymphotactin monomer

Entity

1. Human lymphotactin monomer (polymer, Thiol state: all disulfide bound), 93 monomers, 10271.66 Da Detail

VGSEVSDKRT CVSLTTQRLP VSRIKTYTIT EGSLRAVIFI TKRGLKVCAD PQATWVRDVV RSMDRKSNTR NNMIQTKPTG TQQSTNTAVT LTG

Formula weight

10271.66 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS11:SG	1:CYS48:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.6 %, Completeness (bb): 84.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.6 % (912 of 1053)	94.7 % (517 of 546)	74.7 % (304 of 407)	91.0 % (91 of 100)
Backbone	84.4 % (466 of 552)	100.0 % (188 of 188)	68.6 % (188 of 274)	100.0 % (90 of 90)
Sidechain	90.7 % (534 of 589)	91.9 % (329 of 358)	92.3 % (204 of 221)	10.0 % (1 of 10)
Aromatic	50.0 % (15 of 30)	93.3 % (14 of 15)	0.0 % (0 of 14)	100.0 % (1 of 1)
Methyl	98.3 % (118 of 120)	100.0 % (60 of 60)	96.7 % (58 of 60)

1. human lymphotactin

VGSEVSDKRT CVSLTTQRLP VSRIKTYTIT EGSLRAVIFI TKRGLKVCAD PQATWVRDVV RSMDRKSNTR NNMIQTKPTG TQQSTNTAVT LTG

Show sample condition

Sample

Temperature 283 (±0.2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human lymphotactin	[U-13C; U-15N]		0.5 ~ 1.3 mM
2	NaCl			200 mM

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1J8I, Strand ID: A Detail

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Release date

2001-05-31

Citation

Monomeric solution structure of the prototypical 'C' chemokine lymphotactin
Kuloglu, E.SONAY., McCaslin, D.R., Kitabwalla, M., Pauza, C.DAVID., Markley, J.L., Volkman, B.F.
Biochemistry (2001), 40, 12486-12496, PubMed 11601972 , DOI: , Abstract

Related entities 1. Human lymphotactin monomer, : 1 : 5 : 2 : 10 entities Detail

Interaction partners 1. Human lymphotactin monomer, : 18 interactors Detail

Experiments performed 13 experiments Detail