BMRBj - BMREntryMaster

Found 1 Document (1.371 seconds)

BMRB: 5046

1IDV

NMR Structure of HCV IRES RNA Domain IIIC

Authors

Kaluarachchi, K., Thiviyanathan, V., Rijnbrand, R.A., Lemon, S.M., Gorenstein, D.G.

Assembly

HCV IRES RNA DOMAIN IIIC

Entity

1. HCV IRES RNA DOMAIN IIIC (polymer, Thiol state: not present), 10 monomers, 3270.936 Da Detail

GGGCGUGCCC

Formula weight

3270.936 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 100.0 %, Completeness (bb): 100.0 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	100.0 % (89 of 89)	100.0 % (89 of 89)
Suger, PO4	100.0 % (60 of 60)	100.0 % (60 of 60)
Nucleobase	100.0 % (29 of 29)	100.0 % (29 of 29)
Aromatic	100.0 % (21 of 21)	100.0 % (21 of 21)

1. Hepatitis C Internal Ribosome Entry Site

GGGCGUGCCC

Show sample condition

Sample

Pressure 1 atm, Temperature 283 (±1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
2	sodium phosphate buffer			10 mM
3	KCl			10 mM
4	EDTA			0.05 mM
5	H2O			90 %
6	D2O			10 %

Release date

2005-11-10

Citation

Mutational and structural analysis of stem-loop IIIC of the hepatitis C virus and GB virus B internal ribosome entry sites
Rijnbrand, R.A., Thiviyanathan, V., Kaluarachchi, K., Lemon, S.M., Gorenstein, D.G.
J. Mol. Biol. (2004), 343, 805-817, PubMed 15476802 , DOI 10.1016/j.jmb.2004.08.095 , Abstract

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
2	sodium phosphate buffer			10 mM
3	KCl			10 mM
4	EDTA			0.05 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
8	sodium phosphate buffer			10 mM
9	KCl			10 mM
10	EDTA			0.05 mM
11	D2O			99.996 %

2 DQF-COSY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
2	sodium phosphate buffer			10 mM
3	KCl			10 mM
4	EDTA			0.05 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
8	sodium phosphate buffer			10 mM
9	KCl			10 mM
10	EDTA			0.05 mM
11	D2O			99.996 %

3 2D TOCSY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
2	sodium phosphate buffer			10 mM
3	KCl			10 mM
4	EDTA			0.05 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
8	sodium phosphate buffer			10 mM
9	KCl			10 mM
10	EDTA			0.05 mM
11	D2O			99.996 %

4 31P Hetero-TOCSY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
2	sodium phosphate buffer			10 mM
3	KCl			10 mM
4	EDTA			0.05 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
8	sodium phosphate buffer			10 mM
9	KCl			10 mM
10	EDTA			0.05 mM
11	D2O			99.996 %

5 1H-13C HSQC

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
2	sodium phosphate buffer			10 mM
3	KCl			10 mM
4	EDTA			0.05 mM
5	H2O			90 %
6	D2O			10 %

Sample #2

Pressure 1 atm, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Hepatitis C Internal Ribosome Entry Site			1.0 ~ 2.0 mM
8	sodium phosphate buffer			10 mM
9	KCl			10 mM
10	EDTA			0.05 mM
11	D2O			99.996 %

nullKeywords Hepatitis C RNA, IRES, Stem-loop, Domain IIIC