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Found 1 Document (5.809 seconds)

BMRB: 5053

1IFW

Solution Structure of the Orphan PABC Domain from Saccharomyces cerevisiae Poly(A)-binding Protein

Authors

Kozlov, G., Siddiqui, N., Coillet-Matillon, S., Trempe, J., Ekiel, I., Sprules, T., Gehring, K.

Assembly

C-terminal domain of Pab1p

Entity

1. C-terminal domain of Pab1p (polymer, Thiol state: not present), 92 monomers, 10434.32 Da Detail

GPLGSPRNAN DNNQFYQQKQ RQALGEQLYK KVSAKTSNEE AAGKITGMIL DLPPQEVFPL LESDELFEQH YKEASAAYES FKKEQEQQTE QA

Formula weight

10434.32 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 70.9 %, Completeness (bb): 83.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.9 % (777 of 1096)	80.6 % (469 of 582)	53.4 % (219 of 410)	85.6 % (89 of 104)
Backbone	83.0 % (450 of 542)	94.6 % (174 of 184)	70.8 % (192 of 271)	96.6 % (84 of 87)
Sidechain	64.3 % (412 of 641)	74.1 % (295 of 398)	49.6 % (112 of 226)	29.4 % (5 of 17)
Aromatic	42.1 % (32 of 76)	65.8 % (25 of 38)	18.4 % (7 of 38)
Methyl	66.7 % (48 of 72)	91.7 % (33 of 36)	41.7 % (15 of 36)

1. C-terminal domain of Pab1p

GPLGSPRNAN DNNQFYQQKQ RQALGEQLYK KVSAKTSNEE AAGKITGMIL DLPPQEVFPL LESDELFEQH YKEASAAYES FKKEQEQQTE QA

Show sample condition

Sample #1

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Pab1p	[U-15N]		3.0 mM
2	NaCl			150 mM

Sample #2

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	C-terminal domain of Pab1p			3.0 mM
4	NaCl			150 mM

Sample #3

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	C-terminal domain of Pab1p	[U-13C; U-15N]		3.0 mM
6	NaCl			150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1IFW, Strand ID: A Detail

Coupling constant

68 J values in 1 lists
Temperature 303 (±1) K, pH 6.3 (±0.1) Detail

Release date

2002-08-21

Citation

Solution structure of the orphan PABC domain from Saccharomyces cerevisiae poly(A)-binding protein
Kozlov, G., Siddiqui, N., Coillet-Matillon, S., Trempe, J., Ekiel, I., Sprules, T., Gehring, K.
J. Biol. Chem. (2002), 277, 22822-22828, PubMed 11940585 , DOI 10.1074/jbc.M201230200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P04147 released on 1986-11-01
Title PABP_YEAST Entity Polyadenylate-binding protein, cytoplasmic and nuclear

Related entities 1. C-terminal domain of Pab1p, : 1 : 3 : 26 entities Detail

Interaction partners 1. C-terminal domain of Pab1p, : 181 interactors Detail

More details for 181 interactors identified by 17 citations

Experiments performed 5 experiments Detail

1 2D 1H-1H NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UnityPlus (750 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Pab1p	[U-15N]		3.0 mM
2	NaCl			150 mM

Sample #2

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	C-terminal domain of Pab1p			3.0 mM
4	NaCl			150 mM

Sample #3

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	C-terminal domain of Pab1p	[U-13C; U-15N]		3.0 mM
6	NaCl			150 mM

2 3D 1H-1H-15N NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UnityPlus (750 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Pab1p	[U-15N]		3.0 mM
2	NaCl			150 mM

Sample #2

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	C-terminal domain of Pab1p			3.0 mM
4	NaCl			150 mM

Sample #3

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	C-terminal domain of Pab1p	[U-13C; U-15N]		3.0 mM
6	NaCl			150 mM

3 3D 1H-1H-15N TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UnityPlus (750 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Pab1p	[U-15N]		3.0 mM
2	NaCl			150 mM

Sample #2

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	C-terminal domain of Pab1p			3.0 mM
4	NaCl			150 mM

Sample #3

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	C-terminal domain of Pab1p	[U-13C; U-15N]		3.0 mM
6	NaCl			150 mM

4 3D CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UnityPlus (750 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Pab1p	[U-15N]		3.0 mM
2	NaCl			150 mM

Sample #2

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	C-terminal domain of Pab1p			3.0 mM
4	NaCl			150 mM

Sample #3

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	C-terminal domain of Pab1p	[U-13C; U-15N]		3.0 mM
6	NaCl			150 mM

5 3D HNCACB

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UnityPlus (750 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of Pab1p	[U-15N]		3.0 mM
2	NaCl			150 mM

Sample #2

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	C-terminal domain of Pab1p			3.0 mM
4	NaCl			150 mM

Sample #3

Temperature 303 (±1) K, pH 6.3 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	C-terminal domain of Pab1p	[U-13C; U-15N]		3.0 mM
6	NaCl			150 mM

null

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Found 1 Document (5.809 seconds)

BMRB: 5053

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. C-terminal domain of Pab1p, : 1 : 3 : 26 entities Detail

Interaction partners 1. C-terminal domain of Pab1p, : 181 interactors Detail

Experiments performed 5 experiments Detail

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