BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	45.2 % (502 of 1110)	52.1 % (302 of 580)	31.6 % (137 of 433)	64.9 % (63 of 97)
Backbone	55.5 % (313 of 564)	65.6 % (126 of 192)	44.0 % (125 of 284)	70.5 % (62 of 88)
Sidechain	39.0 % (248 of 636)	45.4 % (176 of 388)	29.7 % (71 of 239)	11.1 % (1 of 9)
Aromatic	9.4 % (6 of 64)	12.5 % (4 of 32)	6.3 % (2 of 32)
Methyl	43.1 % (44 of 102)	66.7 % (34 of 51)	19.6 % (10 of 51)

1. EC005

MGSSHHHHHH SSGLVPRGSH MKNIVPDYRL DMVGEPCPYP AVATLEAMPQ LKKGEILEVV SDCPQSINNI PLDARNHGYT VLDIQQDGPT IRYLIQK

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	EC005	[U-15N]	2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	NaN3		1 mM

Sample #2

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	EC005	[U-15N; U-13C]	2.0 mM
7	phosphate buffer		50 mM
8	NaCl		0.15 M
9	DTT		1 mM
10	NaN3		1 mM

Sample #3

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Concentration
11	EC005	2.0 mM
12	phosphate buffer	50 mM
13	NaCl	0.15 M
14	DTT	1 mM
15	NaN3	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.55 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.55 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.5 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1JE3, Strand ID: A Detail

Release date

2002-08-21

Citation 1

An NMR approach to structural proteomics
Yee, A., Chang, X., Pineda_Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C.H., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 1825-1830, PubMed 11854485 , DOI 10.1073/pnas.042684599 , Abstract

Show more 1 citaion

Citation 2

A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map
Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T.
DNA Res. (1996), 3, 379-392, PubMed 9097040 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. EC005, : 1 : 26 entities Detail

Experiments performed 6 experiments Detail

1 1H-15N TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	EC005	[U-15N]	2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	NaN3		1 mM

Sample #2

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	EC005	[U-15N; U-13C]	2.0 mM
7	phosphate buffer		50 mM
8	NaCl		0.15 M
9	DTT		1 mM
10	NaN3		1 mM

Sample #3

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Concentration
11	EC005	2.0 mM
12	phosphate buffer	50 mM
13	NaCl	0.15 M
14	DTT	1 mM
15	NaN3	1 mM

2 1H-15N NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	EC005	[U-15N]	2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	NaN3		1 mM

Sample #2

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	EC005	[U-15N; U-13C]	2.0 mM
7	phosphate buffer		50 mM
8	NaCl		0.15 M
9	DTT		1 mM
10	NaN3		1 mM

Sample #3

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Concentration
11	EC005	2.0 mM
12	phosphate buffer	50 mM
13	NaCl	0.15 M
14	DTT	1 mM
15	NaN3	1 mM

3 1H-13C TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	EC005	[U-15N]	2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	NaN3		1 mM

Sample #2

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	EC005	[U-15N; U-13C]	2.0 mM
7	phosphate buffer		50 mM
8	NaCl		0.15 M
9	DTT		1 mM
10	NaN3		1 mM

Sample #3

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Concentration
11	EC005	2.0 mM
12	phosphate buffer	50 mM
13	NaCl	0.15 M
14	DTT	1 mM
15	NaN3	1 mM

4 1H-13C NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	EC005	[U-15N]	2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	NaN3		1 mM

Sample #2

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	EC005	[U-15N; U-13C]	2.0 mM
7	phosphate buffer		50 mM
8	NaCl		0.15 M
9	DTT		1 mM
10	NaN3		1 mM

Sample #3

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Concentration
11	EC005	2.0 mM
12	phosphate buffer	50 mM
13	NaCl	0.15 M
14	DTT	1 mM
15	NaN3	1 mM

5 HNCACB

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	EC005	[U-15N]	2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	NaN3		1 mM

Sample #2

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	EC005	[U-15N; U-13C]	2.0 mM
7	phosphate buffer		50 mM
8	NaCl		0.15 M
9	DTT		1 mM
10	NaN3		1 mM

Sample #3

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Concentration
11	EC005	2.0 mM
12	phosphate buffer	50 mM
13	NaCl	0.15 M
14	DTT	1 mM
15	NaN3	1 mM

6 CBCA(CO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	EC005	[U-15N]	2.0 mM
2	phosphate buffer		50 mM
3	NaCl		0.15 M
4	DTT		1 mM
5	NaN3		1 mM

Sample #2

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	EC005	[U-15N; U-13C]	2.0 mM
7	phosphate buffer		50 mM
8	NaCl		0.15 M
9	DTT		1 mM
10	NaN3		1 mM

Sample #3

Temperature 310 (±1) K, pH 7.5 (±0.1)

#	Name	Concentration
11	EC005	2.0 mM
12	phosphate buffer	50 mM
13	NaCl	0.15 M
14	DTT	1 mM
15	NaN3	1 mM

nullKeywords Structural genomics

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BMRB: 5059

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. EC005, : 1 : 26 entities Detail

Experiments performed 6 experiments Detail

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