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Found 1 Document (2.418 seconds)

BMRB: 5065

1IBI

Quail Cysteine and Glycine-rich Protein, NMR, 15 Minimized Model Structures

Authors

Schuler, W., Kloiber, K., Matt, T., Bister, K., Konrat, R.

Assembly

CYSTEINE-RICH PROTEIN 2

Entity

1. CYSTEINE-RICH PROTEIN CRP2(LIM2) (polymer, Thiol state: all other bound), 113 monomers, 12104.59 Da Detail

MDRGERLGIK PESSPSPHRP TTNPNTSKFA QKFGGAEKCS RCGDSVYAAE KVIGAGKPWH KNCFRCAKCG KSLESTTLTE KEGEIYCKGC YAKNFGPKGF GYGQGAGALV HAQ

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

12235.408 Da

Max. entity weight

12104.59 Da

Entity Connection

metal coordination 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS39:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS42:SG	2:ZN1:ZN
3	metal coordination	sing	1:HIS60:ND1	2:ZN1:ZN
4	metal coordination	sing	1:CYS63:SG	2:ZN1:ZN
5	metal coordination	sing	1:CYS66:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS69:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS87:SG	2:ZN1:ZN
8	metal coordination	sing	1:CYS90:SG	2:ZN1:ZN

Source organism

Coturnix japonica

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 56.6 %, Completeness: 49.8 %, Completeness (bb): 52.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	49.8 % (630 of 1264)	48.3 % (322 of 666)	51.4 % (249 of 484)	51.8 % (59 of 114)
Backbone	52.9 % (351 of 664)	51.1 % (120 of 235)	53.6 % (173 of 323)	54.7 % (58 of 106)
Sidechain	47.5 % (331 of 697)	46.9 % (202 of 431)	49.6 % (128 of 258)	12.5 % (1 of 8)
Aromatic	40.6 % (43 of 106)	45.3 % (24 of 53)	34.6 % (18 of 52)	100.0 % (1 of 1)
Methyl	55.6 % (40 of 72)	55.6 % (20 of 36)	55.6 % (20 of 36)

1. CYSTEINE-RICH PROTEIN CRP2(LIM2)

MDRGERLGIK PESSPSPHRP TTNPNTSKFA QKFGGAEKCS RCGDSVYAAE KVIGAGKPWH KNCFRCAKCG KSLESTTLTE KEGEIYCKGC YAKNFGPKGF GYGQGAGALV HAQ

Show sample condition

Sample

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.56 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1IBI, Strand ID: A Detail

Release date

2001-11-13

Citation 1

Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(LIM2): evidence for collective motions in LIM domains
Konrat, R., Schuler, W., Kloiber, K., Matt, T., Bister, K.
Biochemistry (2001), 40, 9596-9604, PubMed 11583159 , DOI: , Abstract

Show more 2 citaions

Citation 2

Solution structure of the carboxyl-terminal LIM domain from quail cysteine-rich protein CRP2
Krautler, B., Konrat, R., Weiskirchen, R., Bister, K.
J. Biol. Chem. (1997), 272, 12001-12007, PubMed 9115265 , DOI: , Abstract

Citation 3

Structure of cysteine- and glycine-rich protein CRP2. Backbone dynamics reveal motional freedom and independent spatial orientation of the lim domains
Bister, K., Konrat, R., Weiskirchen, R., Krautler, B.
J. Biol. Chem. (1998), 273, 23233-23240, PubMed 9722554 , DOI: , Abstract

Hide non-primary 2 citaions

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q05158 released on 1994-02-01
Title CSRP2_COTJA Entity Cysteine and glycine-rich protein 2

Related entities 1. CYSTEINE-RICH PROTEIN CRP2(LIM2), : 1 : 2 : 1 : 1 : 47 entities Detail

Interaction partners 1. CYSTEINE-RICH PROTEIN CRP2(LIM2), : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 15N-HSQC

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

2 3D HNCA

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

3 3D HNCACB

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

4 3D HNCO

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

5 3D CBCA(CO)NH

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

6 3D HCCH-TOCSY

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

7 3D CCH-TOCSY-NNH

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

8 3D 13C-NOESY-HSQC (CA-centered)

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

9 3D 13C-NOESY-HSQC (methyl-centered)

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

10 3D 15N-NOESY-HSQC

Instrument

Model Varian UNITYPLUS (500 MHz)

Sample #1

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
1	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-13C; U-15N]	1.0 ~ 2.0 mM
2	H2O		90 %
3	D2O	[U-2H]	10 %
4	potassium phosphate		20 mM
5	KCl		50 mM
6	dithiothreitol		0.5 mM

Sample #2

Pressure 1 atm, Temperature 299 (±1) K, pH 7.2 (±0.2)

#	Name	Isotope labeling	Concentration
7	CYSTEINE-RICH PROTEIN CRP2(LIM2)	[U-15N]	1.0 ~ 2.0 mM
8	H2O		90 %
9	D2O	[U-2H]	10 %
10	potassium phosphate		20 mM
11	KCl		50 mM
12	dithiothreitol		0.5 mM

nullKeywords LIM domain containing proteins, metal-binding protein

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Found 1 Document (2.418 seconds)

BMRB: 5065

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Chemical shift reference

Chemical shift reference

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. CYSTEINE-RICH PROTEIN CRP2(LIM2), : 1 : 2 : 1 : 1 : 47 entities Detail

Interaction partners 1. CYSTEINE-RICH PROTEIN CRP2(LIM2), : 2 interactors Detail

Experiments performed 10 experiments Detail

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