1H and 15N Chemical Shift Assignments for the Alpha-domain of Mouse Metallothionein-3
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 1:CYS51:SG | 2:CD1:CD |
2 | metal coordination | sing | 1:CYS64:SG | 2:CD1:CD |
3 | metal coordination | sing | 1:CYS66:SG | 2:CD1:CD |
4 | metal coordination | sing | 1:CYS67:SG | 2:CD1:CD |
5 | metal coordination | sing | 1:CYS34:SG | 2:CD1:CD |
6 | metal coordination | sing | 1:CYS35:SG | 2:CD1:CD |
7 | metal coordination | sing | 1:CYS45:SG | 2:CD1:CD |
8 | metal coordination | sing | 1:CYS49:SG | 2:CD1:CD |
9 | metal coordination | sing | 1:CYS38:SG | 2:CD1:CD |
10 | metal coordination | sing | 1:CYS42:SG | 2:CD1:CD |
11 | metal coordination | sing | 1:CYS45:SG | 2:CD1:CD |
12 | metal coordination | sing | 1:CYS67:SG | 2:CD1:CD |
13 | metal coordination | sing | 1:CYS35:SG | 2:CD1:CD |
14 | metal coordination | sing | 1:CYS37:SG | 2:CD1:CD |
15 | metal coordination | sing | 1:CYS38:SG | 2:CD1:CD |
16 | metal coordination | sing | 1:CYS51:SG | 2:CD1:CD |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 54.3 % (232 of 427) | 54.3 % (196 of 361) | 54.5 % (36 of 66) |
Backbone | 56.9 % (115 of 202) | 57.2 % (79 of 138) | 56.3 % (36 of 64) |
Sidechain | 52.0 % (117 of 225) | 52.5 % (117 of 223) | 0.0 % (0 of 2) |
Methyl | 63.6 % (7 of 11) | 63.6 % (7 of 11) |
1. Metallothionein-3
MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV CKGEEGAKAE AEKCSCCQTemperature 283 (±0.1) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Metallothionein-3 | [U-15N] | 1.0 ~ 3.0 mM | |
2 | Tris-HCl | [U-98% 2H] | 20 mM |