BMRBj - BMREntryMaster

Found 1 Document (5.884 seconds)

BMRB: 5070

1JH3

Structure and dynamics of the anticodon-arm binding domain of Bacillus stearothermophilus tyrosyl-tRNA synthetase

Authors

Guijarro, J.INAKI., Pintar, A., Prochnicka-Chalufour, A., Guez, V., Gilquin, B., Bedouelle, H., Delepierre, M.

Assembly

C-terminal domain of tyrosyl-tRNA synthetase

Entity

1. C-terminal domain of tyrosyl-tRNA synthetase (polymer, Thiol state: not present), 107 monomers, 11980.37 Da Detail

ALFSGDIANL TAAEIEQGFK DVPSFVHEGG DVPLVELLVS AGISPSKRQA REDIQNGAIY VNGERLQDVG AILTAEHRLE GRFTVIRRGK KKYYLIRYAL EHHHHHH

Formula weight

11980.37 Da

Source organism

Geobacillus stearothermophilus

Exptl. method

NMR

Refine. method

torsion angle dynamics simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 81.6 %, Completeness (bb): 88.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.6 % (1010 of 1238)	84.4 % (541 of 641)	75.5 % (367 of 486)	91.9 % (102 of 111)
Backbone	88.2 % (561 of 636)	88.7 % (196 of 221)	86.8 % (270 of 311)	91.3 % (95 of 104)
Sidechain	76.4 % (534 of 699)	82.1 % (345 of 420)	66.9 % (182 of 272)	100.0 % (7 of 7)
Aromatic	32.7 % (34 of 104)	61.5 % (32 of 52)	3.8 % (2 of 52)
Methyl	85.4 % (111 of 130)	87.7 % (57 of 65)	83.1 % (54 of 65)

1. TyrRS C-terminal domain

ALFSGDIANL TAAEIEQGFK DVPSFVHEGG DVPLVELLVS AGISPSKRQA REDIQNGAIY VNGERLQDVG AILTAEHRLE GRFTVIRRGK KKYYLIRYAL EHHHHHH

Show sample condition

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.59 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.59 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1JH3, Strand ID: A Detail

Release date

2002-03-28

Citation

Structure and dynamics of the anticodon arm binding domain of Bacillus stearothermophilus Tyrosyl-tRNA synthetase
Guijarro, J.INAKI., Pintar, A., Prochnicka-Chalufour, A., Guez, V., Gilquin, B., Bedouelle, H., Delepierre, M.
Structure (2002), 10, 311-317, PubMed 12005430 , DOI: , Abstract

Related entities 1. C-terminal domain of tyrosyl-tRNA synthetase, : 1 : 4 : 116 entities Detail

Experiments performed 11 experiments Detail

1 HNCA

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

2 HNCO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

3 1H-15N HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

4 1H-13C HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

5 15N-edited NOESY-HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

6 TOCSY-HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

7 CBCA(CO)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

8 HNCACB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

9 H(CC-TOCSY)NNH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

10 C(CC-TOCSY)NNH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

11 13C-edited HCCH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	TyrRS C-terminal domain	[U-15N]	1.2 mM
2	potassium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Temperature 308 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
5	TyrRS C-terminal domain	[U-13C; U-15N]	0.8 mM
6	potassium phosphate		20 mM
7	H2O		90 %
8	D2O		10 %

nullKeywords 1H, 13C and 15N assigned chemical shifts, C-terminal domain of tyrosyl-tRNA synthetase

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Found 1 Document (5.884 seconds)

BMRB: 5070

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. C-terminal domain of tyrosyl-tRNA synthetase, : 1 : 4 : 116 entities Detail

Experiments performed 11 experiments Detail

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