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Found 1 Document (2.224 seconds)

BMRB: 5077

1JJG

1H, 15N, 13C NMR Assignments of M156R Protein from myxoma virus, NESG target OP2

Authors

Ramelot, T.A., Cort, J.R., Yee, A.A., Furong, L., Goshe, M.B., Edwards, A.M., Smith, R.D., Arrowsmith, C.H., Dever, T.E., Kennedy, M.A.

Assembly

M156R

Entity

1. M156R (polymer, Thiol state: all free), 102 monomers, 11973.83 Da Detail

MTVIKPSSRP RPRKNKNIKV NTYRTSAMDL SPGSVHEGIV YFKDGIFKVR LLGYEGHECI LLDYLNYRQD TLDRLKERLV GRVIKTRVVR ADGLYVDLRR FF

Formula weight

11973.83 Da

Source organism

Myxoma virus

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 73.7 %, Completeness (bb): 84.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.7 % (923 of 1252)	69.3 % (457 of 659)	76.9 % (377 of 490)	86.4 % (89 of 103)
Backbone	84.9 % (513 of 604)	84.5 % (175 of 207)	83.3 % (249 of 299)	90.8 % (89 of 98)
Sidechain	65.9 % (490 of 743)	62.4 % (282 of 452)	72.7 % (208 of 286)	0.0 % (0 of 5)
Aromatic	45.8 % (44 of 96)	45.8 % (22 of 48)	45.8 % (22 of 48)
Methyl	91.0 % (111 of 122)	90.2 % (55 of 61)	91.8 % (56 of 61)

1. M156R

MTVIKPSSRP RPRKNKNIKV NTYRTSAMDL SPGSVHEGIV YFKDGIFKVR LLGYEGHECI LLDYLNYRQD TLDRLKERLV GRVIKTRVVR ADGLYVDLRR FF

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1JJG, Strand ID: A Detail

Release date

2002-05-27

Citation 1

Myxoma virus immunomodulatory protein M156R is a structural mimic of eukaryotic translation initiation factor eIF2alpha
Ramelot, T.A., Cort, J.R., Yee, A.A., Furong, L., Goshe, M.B., Edwards, A.M., Smith, R.D., Arrowsmith, C.H., Dever, T.E., Kennedy, M.A.
J. Mol. Biol. (2002), 322, 943-954, PubMed 12367520 , DOI: , Abstract

Show more 1 citaion

Citation 2

An NMR approach to structural proteomics
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C.H., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 1825-1830, PubMed 11854485 , DOI 10.1073/pnas.042684599 , Abstract

Hide non-primary 1 citaion

Related entities 1. M156R, : 1 : 3 entities Detail

Experiments performed 15 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

2 1H-13C HSQC

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

3 15N-edited NOESY-HQSC

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

4 HCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

5 HCCH-TOCSY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

6 CN-NOESY-HSQC

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

7 CBCACOCAHA

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

8 HNCA

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

9 HN(CO)CA

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

10 HNCO

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

11 HNCACB

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

12 CBCA(CO)NH

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

13 CCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

14 HNHA

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

15 4D CC-NOESY

Instrument

#1 Model Varian Inova (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (750 MHz)

#4 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	M156R	[U-15N; U-13C]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	M156R	[U-15N]	1 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

nullKeywords NMR, protein, structure

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Found 1 Document (2.224 seconds)

BMRB: 5077

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. M156R, : 1 : 3 entities Detail

Experiments performed 15 experiments Detail

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