BMRB: 5082

Solution Structure of a K(+)-Channel Blocker from the Scorpion Tityus cambridgei

Authors

Wang, I., Wu, S., Chang, H., Shieh, R., Yu, H., Chen, C.

Assembly

Tc1 monomer

Entity

1. Tc1 monomer (polymer, Thiol state: all disulfide bound), 23 monomers, 2453.976 Da Detail

ACGSCRKKCK GSGKCINGRC KCY

Formula weight

2453.976 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS15:SG
2	disulfide	sing	1:CYS5:SG	1:CYS20:SG
3	disulfide	sing	1:CYS9:SG	1:CYS22:SG

Source organism

Tityus obscurus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.5 %, Completeness (bb): 96.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.5 % (134 of 136)	98.5 % (134 of 136)
Backbone	96.0 % (48 of 50)	96.0 % (48 of 50)
Sidechain	100.0 % (86 of 86)	100.0 % (86 of 86)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (3 of 3)	100.0 % (3 of 3)

1. Tityustoxin alpha-KTx

ACGSCRKKCK GSGKCINGRC KCY

Show sample condition

Sample

Temperature 275 (±1) K, pH 3.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Tityustoxin alpha-KTx			1.5 ~ 2.0 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 1JLZ, Strand ID: A Detail

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Release date

2001-07-16

Citation

Solution structure of a K(+)-channel blocker from the scorpion Tityus cambridgei
Wang, I., Wu, S., Chang, H., Shieh, R., Yu, H., Chen, C.
Protein Sci. (2002), 11, 390-400, PubMed 11790849 , DOI 10.1110/ps.33402 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Tc1 monomer, : 1 : 1 : 1 : 146 entities Detail

Experiments performed 3 experiments Detail