BMRBj - BMREntryMaster

Found 1 Document (1.851 seconds)

BMRB: 5083

1JJJ

1H and 15N chemical shift assignments for human epidermal-type fatty acid-binding protein (E-FABP)

Authors

Gutierrez-Gonzalez, L.H., Ludwig, C., Hohoff, C., Rademacher, M., Hanhoff, T., Rueterjans, H., Spener, F., Luecke, C.

Assembly

Epidermal-type fatty acid binding protein

Entity

1. Epidermal-type fatty acid binding protein (polymer, Thiol state: free and disulfide bound), 135 monomers, 15164.27 Da Detail

MATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI YEKVE

2. STE (non-polymer), 284.477 Da

Total weight

15448.746 Da

Max. entity weight

15164.27 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS120:SG	1:CYS127:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS COMBINED WITH SIMULATED ANNEALING FOLLOWED BY ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 97.4 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	97.4 % (932 of 957)	97.2 % (788 of 811)	98.6 % (144 of 146)
Backbone	97.1 % (402 of 414)	96.4 % (270 of 280)	98.5 % (132 of 134)
Sidechain	97.6 % (530 of 543)	97.6 % (518 of 531)	100.0 % (12 of 12)
Aromatic	100.0 % (44 of 44)	100.0 % (42 of 42)	100.0 % (2 of 2)
Methyl	98.7 % (74 of 75)	98.7 % (74 of 75)

1. Epidermal-type fatty acid binding protein

MATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI YEKVE

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Epidermal-type fatty acid binding protein			1.5 ~ 2.0 mM
2	STEARIC ACID			1.5 ~ 2.0 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Epidermal-type fatty acid binding protein	[U-15N]		1.5 ~ 2.0 mM
4	STEARIC ACID			1.5 ~ 2.0 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1JJJ, Strand ID: A Detail

Release date

2001-07-16

Citation

Solution structure and backbone dynamics of human epidermal-type fatty acid-binding protein (E-FABP)
Gutierrez-Gonzalez, L.H., Ludwig, C., Hohoff, C., Rademacher, M., Hanhoff, T., Rueterjans, H., Spener, F., Luecke, C.
Biochem. J. (2002), 364, 725-737, PubMed 12049637 , DOI 10.1042/BJ20020039 , Abstract

Related entities 1. Epidermal-type fatty acid binding protein, : 1 : 16 : 1 : 1 : 245 entities Detail

Interaction partners 1. Epidermal-type fatty acid binding protein, : 15 interactors Detail

More details for 15 interactors identified by 3 citations

Experiments performed 6 experiments Detail

1 1H-1H TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Epidermal-type fatty acid binding protein			1.5 ~ 2.0 mM
2	STEARIC ACID			1.5 ~ 2.0 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Epidermal-type fatty acid binding protein	[U-15N]		1.5 ~ 2.0 mM
4	STEARIC ACID			1.5 ~ 2.0 mM

2 1H-1H NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Epidermal-type fatty acid binding protein			1.5 ~ 2.0 mM
2	STEARIC ACID			1.5 ~ 2.0 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Epidermal-type fatty acid binding protein	[U-15N]		1.5 ~ 2.0 mM
4	STEARIC ACID			1.5 ~ 2.0 mM

3 1H-15N HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Epidermal-type fatty acid binding protein			1.5 ~ 2.0 mM
2	STEARIC ACID			1.5 ~ 2.0 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Epidermal-type fatty acid binding protein	[U-15N]		1.5 ~ 2.0 mM
4	STEARIC ACID			1.5 ~ 2.0 mM

4 1H-15N HTQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Epidermal-type fatty acid binding protein			1.5 ~ 2.0 mM
2	STEARIC ACID			1.5 ~ 2.0 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Epidermal-type fatty acid binding protein	[U-15N]		1.5 ~ 2.0 mM
4	STEARIC ACID			1.5 ~ 2.0 mM

5 1H-15N TOCSY-HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Epidermal-type fatty acid binding protein			1.5 ~ 2.0 mM
2	STEARIC ACID			1.5 ~ 2.0 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Epidermal-type fatty acid binding protein	[U-15N]		1.5 ~ 2.0 mM
4	STEARIC ACID			1.5 ~ 2.0 mM

6 1H-15N NOESY-HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Epidermal-type fatty acid binding protein			1.5 ~ 2.0 mM
2	STEARIC ACID			1.5 ~ 2.0 mM

Sample #2

Temperature 298 (±0.1) K, pH 5.6 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Epidermal-type fatty acid binding protein	[U-15N]		1.5 ~ 2.0 mM
4	STEARIC ACID			1.5 ~ 2.0 mM

nullKeywords Lipid binding protein, fatty acid carrier

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Found 1 Document (1.851 seconds)

BMRB: 5083

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Related entities 1. Epidermal-type fatty acid binding protein, : 1 : 16 : 1 : 1 : 245 entities Detail

Interaction partners 1. Epidermal-type fatty acid binding protein, : 15 interactors Detail

Experiments performed 6 experiments Detail

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