BMRB: 5086

Reduced recombinant Hydrogenobacter thermophilus cytochrome c-552 expressed in E. coli periplasm

Authors

Karan, E.F., Russell, B.S., Bren, K.L.

Assembly

Hydrogenobacter thermophilus cytochrome c-552

Entity

1. cytochrome c-552 (polymer, Thiol state: all other bound), 80 monomers, 8570.894 Da Detail

NEQLAKQKGC MACHDLKAKK VGPAYADVAK KYAGRKDAVD YLAGKIKKGG SGVWGSVPMP PQNVTDAEAK QLAQWILSIK

2. HEC (non-polymer), 618.503 Da

Total weight

9189.396 Da

Max. entity weight

8570.894 Da

Entity Connection

thioether 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	thioether	sing	1:CYS10:SG	2:HEC1:CAB
2	thioether	sing	1:CYS13:SG	2:HEC1:CAC

Source organism

Hydrogenobacter thermophilus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.8 %, Completeness: 86.2 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	86.2 % (418 of 485)	86.2 % (418 of 485)
Backbone	97.0 % (159 of 164)	97.0 % (159 of 164)
Sidechain	80.7 % (259 of 321)	80.7 % (259 of 321)
Aromatic	73.1 % (19 of 26)	73.1 % (19 of 26)
Methyl	97.6 % (40 of 41)	97.6 % (40 of 41)

1. cytochrome c-552

NEQLAKQKGC MACHDLKAKK VGPAYADVAK KYAGRKDAVD YLAGKIKKGG SGVWGSVPMP PQNVTDAEAK QLAQWILSIK

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 5 (±0.5), Details Reduced with at least a 10-fold molar excess of sodium dithionite.

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c-552			2.0 ~ 3.5 mM
2	heme c			2.0 ~ 3.5 mM
3	sodium acetate	[U-2H]		120 mM
4	sodium dithionite			35.0 ~ 0.0 mM

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Chem. Shift Complete2

Sequence coverage: 15.0 %, Completeness: 45.7 %, Completeness (bb): 54.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	45.7 % (443 of 970)	45.7 % (443 of 970)
Backbone	54.0 % (177 of 328)	54.0 % (177 of 328)
Sidechain	41.4 % (266 of 642)	41.4 % (266 of 642)
Aromatic	36.5 % (19 of 52)	36.5 % (19 of 52)
Methyl	48.8 % (40 of 82)	48.8 % (40 of 82)

1. cytochrome c-552

NEQLAKQKGC MACHDLKAKK VGPAYADVAK KYAGRKDAVD YLAGKIKKGG SGVWGSVPMP PQNVTDAEAK QLAQWILSIK

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 5 (±0.5), Details Reduced with at least a 10-fold molar excess of sodium dithionite.

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c-552			2.0 ~ 3.5 mM
2	heme c			2.0 ~ 3.5 mM
3	sodium acetate	[U-2H]		120 mM
4	sodium dithionite			35.0 ~ 0.0 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1AYG, Strand ID: A Detail

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Release date

2002-08-21

Citation

Characterization of Hydrogenobacter thermophilus cytochromes c(552 )expressed in the cytoplasm and periplasm of Escherichia coli
Karan, E.F., Russell, B.S., Bren, K.L.
J. Biol. Inorg. Chem. (2002), 7, 260-272, PubMed 11935350 , DOI 10.1007/s007750100292 , Abstract

Related entities 1. cytochrome c-552, : 1 : 5 : 45 entities Detail

Experiments performed 2 experiments Detail