BMRBj - BMREntryMaster

Found 1 Document (0.277 seconds)

BMRB: 50902

NMR structure of the antimicrobial RiLK1 peptide in SDS micelles

Authors

Falcigno, L., Agrillo, B., Gogliettino, M., D'Auria, G., Palmieri, G.

Assembly

RiLK1

Entity

1. RiLK1 (polymer, Thiol state: not present), 10 monomers, 1468.796 Da Detail

RLKWVRIWRR

Formula weight

1468.796 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	97.6 % (83 of 85)	97.6 % (83 of 85)
Backbone	95.0 % (19 of 20)	95.0 % (19 of 20)
Sidechain	98.5 % (64 of 65)	98.5 % (64 of 65)
Aromatic	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	83.3 % (5 of 6)	83.3 % (5 of 6)

1. entity 1

RLKWVRIWRR

Show sample condition

Sample

Solvent system SDS-d25 150 mM, 90% H2O, 10% D2O, pH 4.4, Pressure 1 atm, Temperature 298 K, pH 4.4, Details 1mM of peptide

#	Name	Isotope labeling	Concentration
1	RiLK1	natural abundance	0.9 mM
2	D2O	98% D	10 %
3	H2O	natural abundance	90 %
4	SDS	98% D	150 mM

Release date

2021-04-18

Citation

Key Physicochemical Determinants in the Antimicrobial Peptide RiLK1 Promote Amphipathic Structures
Falcigno, L., D'Auria, G., Palmieri, G., Gogliettino, M., Agrillo, B., Tate, R., Dardano, P., Nicolais, L., Balestrieri, M.
Int. J. Mol. Sci. (2021), 22, 10011-10011, PubMed 34576174 , DOI 10.3390/ijms221810011 , Abstract

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	96		100.0 (chain: 1, length: 10)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
3	LYS	HZ1	7.359
3	LYS	HZ2	7.359
3	LYS	HZ3	7.359

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	85	83	97.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	20	19	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	64	98.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	5	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	12	12	100.0

Keywords Antimicrobial peptide; NMR spectroscopy; amphipathic helical structure

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr50902_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (0.277 seconds)

BMRB: 50902

Sample

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail