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Found 1 Document (1.637 seconds)

BMRB: 5096

1JN7

Solution Structure of a CCHH mutant of the ninth CCHC Zinc Finger of U-shaped

Authors

Kowalski, K., Mackay, J.P.

Assembly

U-SHAPED TRANSCRIPTIONAL COFACTOR

Entity

1. U-shaped ninth zinc finger domain (polymer, Thiol state: all other bound), 36 monomers, 4201.800 Da Detail

GSAAEVMKKY CSTCDISFNY VKTYLAHKQF YHKNKP

2. ZN (non-polymer), 65.409 Da

Total weight

4267.209 Da

Max. entity weight

4201.8 Da

Entity Connection

metal coordination 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	2:ZN1:ZN	1:CYS11:SG
2	metal coordination	sing	2:ZN1:ZN	1:CYS14:SG
3	metal coordination	sing	2:ZN1:ZN	1:HIS27:NE2
4	metal coordination	sing	2:ZN1:ZN	1:HIS32:NE2

Source organism

Drosophila melanogaster

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 79.1 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	79.1 % (182 of 230)	79.1 % (182 of 230)
Backbone	93.1 % (67 of 72)	93.1 % (67 of 72)
Sidechain	72.8 % (115 of 158)	72.8 % (115 of 158)
Aromatic	56.7 % (17 of 30)	56.7 % (17 of 30)
Methyl	100.0 % (13 of 13)	100.0 % (13 of 13)

1. U-shaped ninth zinc finger domain

GSAAEVMKKY CSTCDISFNY VKTYLAHKQF YHKNKP

Show sample condition

Sample

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1), Details TCEP is tris(2-carboxyethyl)phosphine, a reducing agent.

#	Name	Concentration
1	U-shaped ninth zinc finger domain	1.0 mM
2	ZnSO4	1.5 mM
3	TCEP	1.5 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1JN7, Strand ID: A Detail

Coupling constant

24 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1) Detail

Release date

2003-06-01

Citation

Characterization of the conserved interaction between GATA and FOG family proteins
Kowalski, K., Liew, C.K., Matthews, J.M., Gell, D.A., Crossley, M., Mackay, J.P.
J. Biol. Chem. (2002), 277, 35720-35729, PubMed 12110675 , DOI 10.1074/jbc.M204663200 , Abstract

Related entities 1. U-shaped ninth zinc finger domain, : 1 : 3 : 18 entities Detail

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1), Details TCEP is tris(2-carboxyethyl)phosphine, a reducing agent.

#	Name	Concentration
1	U-shaped ninth zinc finger domain	1.0 mM
2	ZnSO4	1.5 mM
3	TCEP	1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	U-shaped ninth zinc finger domain	[U-95% 15N]	0.5 mM
5	ZnSO4		0.75 mM
6	TCEP		0.75 mM

2 TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1), Details TCEP is tris(2-carboxyethyl)phosphine, a reducing agent.

#	Name	Concentration
1	U-shaped ninth zinc finger domain	1.0 mM
2	ZnSO4	1.5 mM
3	TCEP	1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	U-shaped ninth zinc finger domain	[U-95% 15N]	0.5 mM
5	ZnSO4		0.75 mM
6	TCEP		0.75 mM

3 DQF-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1), Details TCEP is tris(2-carboxyethyl)phosphine, a reducing agent.

#	Name	Concentration
1	U-shaped ninth zinc finger domain	1.0 mM
2	ZnSO4	1.5 mM
3	TCEP	1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	U-shaped ninth zinc finger domain	[U-95% 15N]	0.5 mM
5	ZnSO4		0.75 mM
6	TCEP		0.75 mM

4 E-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1), Details TCEP is tris(2-carboxyethyl)phosphine, a reducing agent.

#	Name	Concentration
1	U-shaped ninth zinc finger domain	1.0 mM
2	ZnSO4	1.5 mM
3	TCEP	1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	U-shaped ninth zinc finger domain	[U-95% 15N]	0.5 mM
5	ZnSO4		0.75 mM
6	TCEP		0.75 mM

5 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1), Details TCEP is tris(2-carboxyethyl)phosphine, a reducing agent.

#	Name	Concentration
1	U-shaped ninth zinc finger domain	1.0 mM
2	ZnSO4	1.5 mM
3	TCEP	1.5 mM

Sample #2

Pressure 1 atm, Temperature 293 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
4	U-shaped ninth zinc finger domain	[U-95% 15N]	0.5 mM
5	ZnSO4		0.75 mM
6	TCEP		0.75 mM

nullKeywords protein-protein interaction, transcription, zinc finger

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Found 1 Document (1.637 seconds)

BMRB: 5096

Sample

Related entities 1. U-shaped ninth zinc finger domain, : 1 : 3 : 18 entities Detail

Experiments performed 5 experiments Detail

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Sample #1

Sample #2

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