BMRB: 51127

A functional SNP regulates E-cadherin expression by dynamically remodeling the 3D structure of a promoter-associated non-coding RNA transcript

Authors

Sharma, S.S.S., Varani, G.G.V.

Assembly

paRNA

Entity

1. paRNA (polymer, Thiol state: not present), 94 monomers, 30550.18 Da Detail

GGACCCAUAA CCCACCUAGA CCCUAGCUUC GGCUAGAGGG UCAACGCGAA AGCGAGGCCG GGUGGGCGGG UACGUCCGCC CUGGGGAGGG GUCC

Formula weight

30550.18 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.3 %, Completeness (bb): 78.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	81.3 % (1213 of 1492)	79.6 % (654 of 822)	85.1 % (530 of 623)	61.7 % (29 of 47)
Suger, PO4	78.2 % (809 of 1034)	76.6 % (432 of 564)	80.2 % (377 of 470)
Nucleobase	88.2 % (404 of 458)	86.0 % (222 of 258)	100.0 % (153 of 153)	61.7 % (29 of 47)
Aromatic	91.5 % (366 of 400)	92.0 % (184 of 200)	100.0 % (153 of 153)	61.7 % (29 of 47)

1. entity 1

GGACCCAUAA CCCACCUAGA CCCUAGCUUC GGCUAGAGGG UCAACGCGAA AGCGAGGCCG GGUGGGCGGG UACGUCCGCC CUGGGGAGGG GUCC

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	paRNA	[U-99% 13C; U-99% 15N]		0.75 mM
2	paRNA	[U-99% 13C; U-99% 15N]		0.75 mM
3	Sodium phosphate	natural abundance		20 mM

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Release date

2021-10-04

Citation

A functional SNP regulates E-cadherin expression by dynamically remodeling the 3D structure of a promoter-associated non-coding RNA transcript
Sharma, S., Pisignano, G., Merulla, J., Catapano, C.V., Varani, G.
Nucleic Acids Res. (2022), 50, 11331-11343, PubMed 36243981 , DOI 10.1093/nar/gkac875 , Abstract

Experiments performed 6 experiments Detail