BMRB: 5120

1H Chemical Shift Assigments for the mEGF/TGFalpha44-50 chimeric growth factor

Authors

Chamberlin, S.G., Brennan, L., Puddicombe, S.M., Davies, D.E., Turner, D.L.

Assembly

mEGF/TGFalpha44-50 chimera

Entity

1. mEGF/TGFalpha44-50 chimera (polymer, Thiol state: all disulfide bound), 49 monomers, 5254.759 Da Detail

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCEHADLLA

Formula weight

5254.759 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS20:SG
2	disulfide	sing	1:CYS14:SG	1:CYS31:SG
3	disulfide	sing	1:CYS33:SG	1:CYS42:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS WITH SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.3 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	88.3 % (226 of 256)	88.3 % (226 of 256)
Backbone	97.1 % (99 of 102)	97.1 % (99 of 102)
Sidechain	82.5 % (127 of 154)	82.5 % (127 of 154)
Aromatic	70.8 % (17 of 24)	70.8 % (17 of 24)
Methyl	89.5 % (17 of 19)	89.5 % (17 of 19)

1. mEGF/TGFalpha44-50 chimera

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCEHADLLA

Show sample condition

Sample

Temperature 298 (±1) K, pH 3.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	mEGF/TGFalpha44-50 chimera			3 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 1GK5, Strand ID: A Detail

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Release date

2001-08-22

Citation 1

Solution structure of the mEGF/TGFalpha44-50 chimeric growth factor
Chamberlin, S.G., Brennan, L., Puddicombe, S.M., Davies, D.E., Turner, D.L.
Eur. J. Biochem. (2001), 268, 6247-6255, PubMed 11733021 , DOI: , Abstract

Show more 1 citaion

Citation 2

The interaction of an epidermal growth factor/transforming growth factor alpha tail chimera with the human epidermal growth factor receptor reveals unexpected complexities
Puddicombe, S.M., Wood, L., Chamberlin, S.G., Davies, D.E.
J. Biol. Chem. (1996), 271, 30392-30397, PubMed 8940002 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. mEGF/TGFalpha44-50 chimera, : 1 : 170 entities Detail

Interaction partners 1. mEGF/TGFalpha44-50 chimera, : 5 interactors Detail

Experiments performed 3 experiments Detail