BMRB: 5122

In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp. PCC6803 DnaB Mini-intein

Authors

Williams, N.K., Prosselkov, P., Liepinsh, E., Line, I., Sharipo, A., Littler, D.R., Curmi, P.M.G., Otting, G., Dixon, N.E.

Assembly

cyclic N-terminal domain of DnaB helicase

Entity

1. cyclic N-terminal domain of DnaB helicase (polymer, Thiol state: not present), 122 monomers, 13576.98 Da Detail

KVPPHSIEAE QSVLGGLMLD NERWDDVAER VVADDFYTRP HRHIFTEMAR LQESGSPIDL ITLAESLERQ GQLDSVGGFA YLAELSKNTP SAANISAYAD IVRERAVVRE MISTRESGSI EF

Formula weight

13576.98 Da

Entity Connection

peptide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	peptide	sing	1:LYS1:N	1:PHE122:C

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING IN TORSION ANGLE SPACE

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.6 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	96.6 % (807 of 835)	96.5 % (685 of 710)	97.6 % (122 of 125)
Backbone	98.6 % (358 of 363)	99.2 % (244 of 246)	97.4 % (114 of 117)
Sidechain	95.1 % (449 of 472)	95.0 % (441 of 464)	100.0 % (8 of 8)
Aromatic	95.6 % (43 of 45)	95.5 % (42 of 44)	100.0 % (1 of 1)
Methyl	100.0 % (71 of 71)	100.0 % (71 of 71)

1. N-terminal Domain of E. coli DnaB Helicase

KVPPHSIEAE QSVLGGLMLD NERWDDVAER VVADDFYTRP HRHIFTEMAR LQESGSPIDL ITLAESLERQ GQLDSVGGFA YLAELSKNTP SAANISAYAD IVRERAVVRE MISTRESGSI EF

Show sample condition

Sample #1

Temperature 304 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	N-terminal Domain of E. coli DnaB Helicase			2.1 mM

Sample #2

Temperature 304 (±1) K

#	Name	Isotope labeling	Type	Concentration
2	N-terminal Domain of E. coli DnaB Helicase	[U-95% 15N]		2.1 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1JWE, Strand ID: A Detail

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Release date

2001-08-23

Citation

In vivo protein cyclization promoted by a circularly permuted Synechocystis sp. PCC6803 DnaB mini-intein
Williams, N.K., Prosselkov, P., Liepinsh, E., Line, I., Sharipo, A., Littler, D.R., Curmi, P.M.G., Otting, G., Dixon, N.E.
J. Biol. Chem. (2002), 277, 7790-7798, PubMed 11742000 , DOI 10.1074/jbc.M110303200 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. cyclic N-terminal domain of DnaB helicase, : 1 : 4 : 40 entities Detail

Interaction partners 1. cyclic N-terminal domain of DnaB helicase, : 20 interactors Detail

Experiments performed 4 experiments Detail