BMRBj - BMREntryMaster

Found 1 Document (0.714 seconds)

BMRB: 51222

6O20

Calmodulin bound to the beta-subunit of the retinal cyclic nucleotide-gated cation channel

Authors

Bej, A., Ames, J.B.

Assembly

Calmodulin

Entity

1. Calmodulin, entity 1 (polymer, Thiol state: not present), 149 monomers, 16837.41 Da Detail

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK

2. Calmodulin, entity CA (non-polymer), 40.078 × 4 Da

Total weight

16997.723 Da

Max. entity weight

16837.41 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 69.5 %, Completeness (bb): 87.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.5 % (1174 of 1689)	67.2 % (587 of 874)	71.1 % (462 of 650)	75.8 % (125 of 165)
Backbone	87.5 % (779 of 890)	87.3 % (268 of 307)	88.5 % (386 of 436)	85.0 % (125 of 147)
Sidechain	55.5 % (520 of 937)	56.4 % (320 of 567)	56.8 % (200 of 352)	0.0 % (0 of 18)
Aromatic	30.0 % (30 of 100)	60.0 % (30 of 50)	0.0 % (0 of 50)
Methyl	83.1 % (118 of 142)	84.5 % (60 of 71)	81.7 % (58 of 71)

1. entity 1

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
5	Tris-d11	natural abundance	20 mM
6	CaCl2	natural abundance	1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	Calmodulin	[U-10% 13C; U-99% 15N]	0.5 mM
8	Tris-d11	natural abundance	20 mM
9	CaCl2	natural abundance	1 mM

Release date

2021-12-05

Citation

Chemical shift assignments of calmodulin bound to the β-subunit of a retinal cyclic nucleotide-gated channel (CNGB1)
Bej, A., Ames, J.B.
Biomol. NMR Assign. (2022), 16, 147-151, PubMed 35107779 , DOI 10.1007/s12104-022-10072-9 , Abstract

Related entities 1. Calmodulin, entity 1, : 1 : 110 : 6 : 4 : 178 entities Detail

Interaction partners 1. Calmodulin, entity 1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

2 3D HNCO

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

3 3D HNCA

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

4 3D HNCACB

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

5 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

6 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

7 3D HBHANH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

8 3D C(CO)NH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

9 3D H(CCO)NH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris-d11	natural abundance	20 mM
3	CaCl2	natural abundance	1 mM

10 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
5	Tris-d11	natural abundance	20 mM
6	CaCl2	natural abundance	1 mM

11 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	Calmodulin	[U-10% 13C; U-99% 15N]	0.5 mM
8	Tris-d11	natural abundance	20 mM
9	CaCl2	natural abundance	1 mM

12 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
5	Tris-d11	natural abundance	20 mM
6	CaCl2	natural abundance	1 mM

13 3D 13C-separated NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
5	Tris-d11	natural abundance	20 mM
6	CaCl2	natural abundance	1 mM

14 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
5	Tris-d11	natural abundance	20 mM
6	CaCl2	natural abundance	1 mM

15 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	0.5 mM
5	Tris-d11	natural abundance	20 mM
6	CaCl2	natural abundance	1 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr51222_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr51222_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1285		95.3 (chain: 1, length: 149)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 149, Sequence coverage: 95.3%
- Total number of assignments
  - ¹H chemical shifts: 704
  - ¹³C chemical shifts: 459
  - ¹⁵N chemical shifts: 122
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	307	264	86.0
¹³C chemical shifts	298	259	86.9
¹⁵N chemical shifts	147	122	83.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	81	64	79.0
¹³C chemical shifts	81	61	75.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	874	576	65.9
¹³C chemical shifts	650	459	70.6
¹⁵N chemical shifts	165	122	73.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	567	312	55.0
¹³C chemical shifts	352	200	56.8
¹⁵N chemical shifts	18	0	0.0

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Found 1 Document (0.714 seconds)

BMRB: 51222

Sample #1

Sample #2

Sample #3

Related entities 1. Calmodulin, entity 1, : 1 : 110 : 6 : 4 : 178 entities Detail

Interaction partners 1. Calmodulin, entity 1, : 1 interactors Detail

Experiments performed 15 experiments Detail

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Chemical shift validation 4 contents Detail