BMRB: 51310

Encoded Conformational Dynamics of the HIV Splice Site A3 Regulatory Locus: Implications for differential binding of hnRNP Splicing Auxiliary Factors

Authors

Chiu, L., Jain, N., Sugarman, A., Tolbert, B.

Assembly

entity 1

Entity

1. entity 1 (polymer, Thiol state: not present), 43 monomers, 13811.09 Da Detail

GGUGCUGUUU AUCCAUUUCA GAAUUGGGUG UCGACAUAGC ACC

Formula weight

13811.09 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 36.4 %, Completeness (bb): 33.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	36.4 % (137 of 376)	36.4 % (137 of 376)
Suger, PO4	33.3 % (86 of 258)	33.3 % (86 of 258)
Nucleobase	43.2 % (51 of 118)	43.2 % (51 of 118)
Aromatic	51.0 % (51 of 100)	51.0 % (51 of 100)

1. entity 1

GGUGCUGUUU AUCCAUUUCA GAAUUGGGUG UCGACAUAGC ACC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	ESS2p Equimolar mix	selective deuteration		450 uM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
2	ESS2p fully protonated	natural abundance		400 uM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
3	ESS2p_N15_HSQC	[U-100% 15N] on GTP and UTP		300 uM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
4	ESS2p_uniform_N15	[U-100% 13C; U-100% 15N]		300 uM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	ESS2p_uniform_N15_C13	[U-100% 13C; U-100% 15N] on ATP and GTP		200 uM

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Release date

2022-02-14

Citation

Encoded Conformational Dynamics of the HIV Splice Site A3 Regulatory Locus: Implications for Differential Binding of hnRNP Splicing Auxiliary Factors
Chiu, L.Y., Emery, A., Jain, N., Sugarman, A., Kendrick, N., Luo, L., Ford, W., Swanstrom, R., Tolbert, B.S.
J. Mol. Biol. (2022), 434, 167728-167728, PubMed 35870649 , DOI 10.1016/j.jmb.2022.167728 , Abstract

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail