BMRB: 5134

Solution Structure of dAAUAA DNA Bulge

Authors

Gollmick, F.A., Lorenz, M., Dornberger, U., von Langen, J., Diekmann, S., Fritzsche, H.

Assembly

dAAUAA DNA Bulge: 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3'

Entity

1. 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3' (polymer, Thiol state: not present), 17 monomers, 5269.358 Da Detail

GCATCGAAXA AGCTACG

2. 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3' (polymer, Thiol state: not present), 12 monomers, 3726.364 Da Detail

CGTAGCCGAT GC

Total weight

8995.722 Da

Max. entity weight

5269.358 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 55.2 %, Completeness: 48.3 %, Completeness (bb): 49.6 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	48.3 % (145 of 300)	47.8 % (130 of 272)	53.6 % (15 of 28)
Suger, PO4	49.6 % (111 of 224)	49.0 % (96 of 196)	53.6 % (15 of 28)
Nucleobase	44.7 % (34 of 76)	44.7 % (34 of 76)
Aromatic	57.1 % (32 of 56)	57.1 % (32 of 56)
Methyl	50.0 % (2 of 4)	50.0 % (2 of 4)

1. 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3'

GCATCGAAXA AGCTACG

2. 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'

CGTAGCCGAT GC

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3'			3.8 mM
2	5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'			3.8 mM
3	phosphate buffer			10 mM
4	NaCl			100 mM
5	EDTA			0.05 mM
6	D2O			100 %

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Chem. Shift Complete2

Sequence coverage: 41.4 %, Completeness: 42.8 %, Completeness (bb): 44.0 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	42.8 % (257 of 600)	42.5 % (231 of 544)	46.4 % (26 of 56)
Suger, PO4	44.0 % (197 of 448)	43.6 % (171 of 392)	46.4 % (26 of 56)
Nucleobase	39.5 % (60 of 152)	39.5 % (60 of 152)
Aromatic	50.0 % (56 of 112)	50.0 % (56 of 112)
Methyl	50.0 % (4 of 8)	50.0 % (4 of 8)

1. 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3'

GCATCGAAXA AGCTACG

2. 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'

CGTAGCCGAT GC

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*UP*AP*AP*GP*CP*TP*AP*CP*G)-3'			3.8 mM
2	5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'			3.8 mM
3	phosphate buffer			10 mM
4	NaCl			100 mM
5	EDTA			0.05 mM
6	D2O			100 %

Hide sample codition

Release date

2015-08-04

Citation

Solution structure of dAATAA and dAAUAA DNA bulges
Gollmick, F.A., Lorenz, M., Dornberger, U., von Langen, J., Diekmann, S., Fritzsche, H.
Nucleic Acids Res. (2002), 30, 2669-2677, PubMed 12060684 , Abstract

Experiments performed 3 experiments Detail