BMRB: 5135

Solution Structure of dAATAA DNA Bulge

Authors

Gollmick, F.A., Lorenz, M., Dornberger, U., von Langen, J., Diekmann, S., Fritzsche, H.

Assembly

dAATAA DNA Bulge: 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*TP*AP*AP*GP*CP*TP*AP*CP*G)-3'

Entity

1. 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*TP*AP*AP*GP*CP*TP*AP*CP*G)-3' (polymer, Thiol state: not present), 17 monomers, 5283.384 Da Detail

GCATCGAATA AGCTACG

2. 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3' (polymer, Thiol state: not present), 12 monomers, 3726.364 Da Detail

CGTAGCCGAT GC

Total weight

9009.748 Da

Max. entity weight

5283.384 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 51.7 %, Completeness: 51.7 %, Completeness (bb): 51.7 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	31P
All	51.7 % (15 of 29)	51.7 % (15 of 29)
Suger, PO4	51.7 % (15 of 29)	51.7 % (15 of 29)

1. 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*TP*AP*AP*GP*CP*TP*AP*CP*G)-3'

GCATCGAATA AGCTACG

2. 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'

CGTAGCCGAT GC

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*TP*AP*AP*GP*CP*TP*AP*CP*G)-3'			1.6 mM
2	5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'			1.6 mM
3	phosphate buffer			10 mM
4	NaCl			100 mM
5	EDTA			0.05 mM
6	D2O			100 %

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 37.9 %, Completeness: 44.8 %, Completeness (bb): 44.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
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Polymer type: polydeoxyribonucleotide

	Total	31P
All	44.8 % (26 of 58)	44.8 % (26 of 58)
Suger, PO4	44.8 % (26 of 58)	44.8 % (26 of 58)

1. 5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*TP*AP*AP*GP*CP*TP*AP*CP*G)-3'

GCATCGAATA AGCTACG

2. 5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'

CGTAGCCGAT GC

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	5'-D(*GP*CP*AP*TP*CP*GP*AP*AP*TP*AP*AP*GP*CP*TP*AP*CP*G)-3'			1.6 mM
2	5'-D(*CP*GP*TP*AP*GP*CP*CP*GP*AP*TP*GP*C)-3'			1.6 mM
3	phosphate buffer			10 mM
4	NaCl			100 mM
5	EDTA			0.05 mM
6	D2O			100 %

Hide sample codition

Release date

2002-08-22

Citation

Solution structure of dAATAA and dAAUAA DNA bulges
Gollmick, F.A., Lorenz, M., Dornberger, U., von Langen, J., Diekmann, S., Fritzsche, H.
Nucleic Acids Res. (2002), 30, 2669-2677, PubMed 12060684 , DOI: , Abstract

Entries sharing articles BMRB: 1 entries Detail

Experiments performed 3 experiments Detail