BMRB: 5141

Sequence-specific resonance assignments of the N-terminal, 105-residue KaiC-interacting domain of SasA, a protein necessary for a robust circadian rhythm in Synechococcus elongatus

Authors

Klewer, D.A., Williams, S.B., Golden, S.S., LiWang, A.C.

Assembly

SasA N-terminus

Entity

1. SasA N-terminus (polymer, Thiol state: not present), 105 monomers, 11476.19 Da Detail

GSSLSPQALA QPLLLQLFVD TRPLSQHIVQ RVKNILAAVE ATVPISLQVI NVADQPQLVE YYRLVVTPAL VKIGPGSRQV LSGIDLTDQL ANQLPQWLVQ QEGIF

Formula weight

11476.19 Da

Source organism

Synechococcus elongatus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, slow refinement in the presence of database potentials

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 82.4 %, Completeness (bb): 82.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.4 % (1023 of 1241)	87.2 % (560 of 642)	74.4 % (360 of 484)	89.6 % (103 of 115)
Backbone	82.2 % (505 of 614)	94.7 % (196 of 207)	69.4 % (215 of 310)	96.9 % (94 of 97)
Sidechain	84.9 % (617 of 727)	83.7 % (364 of 435)	89.1 % (244 of 274)	50.0 % (9 of 18)
Aromatic	76.9 % (40 of 52)	76.9 % (20 of 26)	76.0 % (19 of 25)	100.0 % (1 of 1)
Methyl	79.7 % (137 of 172)	80.2 % (69 of 86)	79.1 % (68 of 86)

1. SasA

GSSLSPQALA QPLLLQLFVD TRPLSQHIVQ RVKNILAAVE ATVPISLQVI NVADQPQLVE YYRLVVTPAL VKIGPGSRQV LSGIDLTDQL ANQLPQWLVQ QEGIF

Show sample condition

Sample

Temperature 298 (±0.05) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SasA	[U-99% 13C; U-99% 15N]		0.6 mM

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 25 models in PDB: 1T4Z, Strand ID: A Detail

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Release date

2002-11-03

Citation

Sequence-specific resonance assignments of the N-terminal, 105-residue KaiC-interacting domain of SasA, a protein necessary for a robust circadian rhythm in Synechococcus elongatus
Klewer, D.A., Williams, S.B., Golden, S.S., LiWang, A.C.
J. Biomol. NMR (2002), 24, 77-78, PubMed 12449424 , DOI: , Abstract

Related entities 1. SasA N-terminus, : 1 : 1 : 1 : 30 entities Detail

Interaction partners 1. SasA N-terminus, : 4 interactors Detail

Experiments performed 8 experiments Detail