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Found 1 Document (0.586 seconds)

BMRB: 51447

6O20

Calmodulin bound to the C-terminal calmodulin-binding site of the beta-subunit of the retinal cyclic nucleotide-gated cation channel

Authors

Bej, A., Ames, J.B.

Assembly

Calcium-bound calmodulin

Entity

1. Calcium-bound calmodulin, entity 1 (polymer, Thiol state: not present), 149 monomers, 16837.41 Da Detail

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK

2. Calcium-bound calmodulin, entity CA (non-polymer), 40.078 × 4 Da

Total weight

16997.723 Da

Max. entity weight

16837.41 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 77.1 %, Completeness (bb): 91.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.1 % (1303 of 1689)	75.6 % (661 of 874)	77.5 % (504 of 650)	83.6 % (138 of 165)
Backbone	91.7 % (816 of 890)	91.9 % (282 of 307)	91.1 % (397 of 436)	93.2 % (137 of 147)
Sidechain	65.2 % (611 of 937)	66.5 % (377 of 567)	66.5 % (234 of 352)	0.0 % (0 of 18)
Aromatic	34.0 % (34 of 100)	68.0 % (34 of 50)	0.0 % (0 of 50)
Methyl	92.3 % (131 of 142)	90.1 % (64 of 71)	94.4 % (67 of 71)

1. entity 1

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
5	TRIS	[U-99% 2H]	20 mM
6	CaCl2	natural abundance	1 mM

Release date

2022-05-16

Citation

NMR Structures of Calmodulin Bound to Two Separate Regulatory Sites in the Retinal Cyclic Nucleotide-Gated Channel
Bej, A., Ames, J.B.
Biochemistry (2022), 61, 1955-1965, PubMed 36070238 , DOI 10.1021/acs.biochem.2c00378 , Abstract

Related entities 1. Calcium-bound calmodulin, entity 1, : 1 : 110 : 6 : 4 : 178 entities Detail

Interaction partners 1. Calcium-bound calmodulin, entity 1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

2 3D HNCACB

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

3 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

4 3D HNCO

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

5 3D HBHANH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

6 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

7 3D C(CO)NH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

8 3D H(CCO)NH

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

9 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
5	TRIS	[U-99% 2H]	20 mM
6	CaCl2	natural abundance	1 mM

10 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
5	TRIS	[U-99% 2H]	20 mM
6	CaCl2	natural abundance	1 mM

11 3D 13C-separated NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
4	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
5	TRIS	[U-99% 2H]	20 mM
6	CaCl2	natural abundance	1 mM

12 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

13 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]	400 uM
2	TRIS	[U-99% 2H]	20 mM
3	CaCl2	natural abundance	1 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr51447_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr51447_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1450		96.6 (chain: 1, length: 149)
1	Chemical shift references	chem_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 149, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 814
  - ¹³C chemical shifts: 498
  - ¹⁵N chemical shifts: 138
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	307	286	93.2
¹³C chemical shifts	298	266	89.3
¹⁵N chemical shifts	147	138	93.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	874	664	76.0
¹³C chemical shifts	650	498	76.6
¹⁵N chemical shifts	165	138	83.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	567	378	66.7
¹³C chemical shifts	352	232	65.9
¹⁵N chemical shifts	18	0	0.0

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Found 1 Document (0.586 seconds)

BMRB: 51447

Sample #1

Sample #2

Related entities 1. Calcium-bound calmodulin, entity 1, : 1 : 110 : 6 : 4 : 178 entities Detail

Interaction partners 1. Calcium-bound calmodulin, entity 1, : 1 interactors Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 4 contents Detail