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Found 1 Document (1.971 seconds)

BMRB: 5145

1LG4

NMR Structure of the human Doppel Protein

Authors

Luhrs, T., Riek, R., Guntert, P., Wuthrich, K.

Assembly

human Doppel

Entity

1. human Doppel (polymer, Thiol state: not reported), 131 monomers, 14966.62 Da Detail

GSVQTRGIKH RIKWNRKALP STAQITEAQV AENRPGAFIK QGRKLDIDFG AEGNRYYEAN YWQFPDGIHY NGCSEANVTK EAFVTGCINA TQAANQGEFQ KPDNKLHQQV LWRLVQELCS LKHCEFWLER G

Formula weight

14966.62 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 87.6 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.6 % (1367 of 1560)	96.7 % (791 of 818)	72.9 % (431 of 591)	96.0 % (145 of 151)
Backbone	82.5 % (642 of 778)	97.4 % (262 of 269)	67.5 % (258 of 382)	96.1 % (122 of 127)
Sidechain	93.3 % (842 of 902)	96.4 % (529 of 549)	88.1 % (290 of 329)	95.8 % (23 of 24)
Aromatic	78.8 % (123 of 156)	97.4 % (76 of 78)	58.1 % (43 of 74)	100.0 % (4 of 4)
Methyl	100.0 % (120 of 120)	100.0 % (60 of 60)	100.0 % (60 of 60)

1. Doppel Protein

GSVQTRGIKH RIKWNRKALP STAQITEAQV AENRPGAFIK QGRKLDIDFG AEGNRYYEAN YWQFPDGIHY NGCSEANVTK EAFVTGCINA TQAANQGEFQ KPDNKLHQQV LWRLVQELCS LKHCEFWLER G

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1LG4, Strand ID: A Detail

Release date

2003-02-24

Citation 1

NMR structure of the human doppel protein
Luhrs, T., Riek, R., Guntert, P., Wuthrich, K.
J. Mol. Biol. (2003), 326, 1549-1557, PubMed 12595265 , DOI: , Abstract

Show more 1 citaion

Citation 2

Ataxia in prion protein (PrP)-deficient mice is associated with upregulation of the novel PrP-like protein doppel
Moore, R.C., Lee, I.Y., Silverman, G.L., Harrison, P.M., Strome, R., Heinrich, C., Karunaratne, A., Pasternak, S.H., Chishti, M.A., Liang, Y., Mastrangelo, P., Wang, K., Smit, A.F., Katamine, S., Carlson, G.A., Cohen, F.E., Prusiner, S.B., Melton, D.W., Tremblay, P., Hood, L.E., Westaway, D.
J. Mol. Biol. (1999), 292, 797-817, PubMed 10525406 , DOI 10.1006/jmbi.1999.3108 , Abstract

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q9QUG3 released on 2002-09-19
    Title PRND_MOUSE Entity Prion-like protein doppel
  Swiss-Prot: Q9UKY0 released on 2002-09-19
    Title PRND_HUMAN Entity Prion-like protein doppel

Related entities 1. human Doppel, : 1 : 7 entities Detail

Interaction partners 1. human Doppel, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 1H-15N HSQC

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

2 1H-13C HSQC

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

3 HNCA

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

4 HNCACB

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

5 HN(CO)CACB

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

6 1H-15N 3D-TOCSY

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

7 HCCH COSY

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

8 1H-15N 3D-NOESY

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

9 1H-13C 3D-NOESY

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

10 1H-1H 2D-NOESY

Instrument

Model na unknown (na MHz) Details spectrometer information not available

Sample #1

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Doppel Protein	[U-15N; U-13C]		1.5 mM

Sample #2

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Doppel Protein			2.0 mM

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Found 1 Document (1.971 seconds)

BMRB: 5145

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. human Doppel, : 1 : 7 entities Detail

Interaction partners 1. human Doppel, : 1 interactors Detail

Experiments performed 10 experiments Detail

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