BMRB: 5151

Designed Protein G Core Variants Fold to Native-like Structures: Sequence Selection by ORBIT Tolerates Variation in Backbone Specification

Authors

Ross, S.A., Sarisky, C.A., Su, A., Mayo, S.L.

Assembly

IgG-binding (B1) domain of Streptococcal protein G

Entity

1. IgG-binding (B1) domain of Streptococcal protein G (polymer, Thiol state: not present), 57 monomers, 6294.863 Da Detail

MTTFKLIING KTLKGETTTE AVDAATAEKV FKQYANDNGI DGEWTYDDAT KTFTVTE

Formula weight

6294.863 Da

Source organism

Streptococcus sp.

Exptl. method

NMR

Refine. method

Distance Geometry and Simulated Annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.7 %, Completeness (bb): 99.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.7 % (310 of 324)	95.7 % (310 of 324)
Backbone	99.2 % (117 of 118)	99.2 % (117 of 118)
Sidechain	93.7 % (193 of 206)	93.7 % (193 of 206)
Aromatic	86.2 % (25 of 29)	86.2 % (25 of 29)
Methyl	100.0 % (34 of 34)	100.0 % (34 of 34)

1. IMMUNOGLOBULIN G BINDING PROTEIN G

MTTFKLIING KTLKGETTTE AVDAATAEKV FKQYANDNGI DGEWTYDDAT KTFTVTE

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Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	IMMUNOGLOBULIN G BINDING PROTEIN G			3 mM
2	sodium phosphate			50 mM
3	H2O			90 %
4	D2O			10 %

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Protein Blocks Logo

Calculated from 40 models in PDB: 1FD6, Strand ID: A Detail

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Release date

2001-10-16

Citation

Designed protein G core variants fold to native-like structures: sequence selection by ORBIT tolerates variation in backbone specification
Ross, S.A., Sarisky, C.A., Su, A., Mayo, S.L.
Protein Sci. (2001), 10, 450-454, PubMed 11266631 , DOI 10.1110/ps.32501 , Abstract

Related entities 1. IgG-binding (B1) domain of Streptococcal protein G, : 1 : 4 : 3 : 97 entities Detail

Interaction partners 1. IgG-binding (B1) domain of Streptococcal protein G, : 1 interactors Detail

Experiments performed 4 experiments Detail