BMRB: 5154

Backbone dynamics of free N-TIMP-1 in solution

Authors

Gao, G., Semenchenko, V., Arumugam, S., Van Doren, S.R.

Assembly

N-terminal domain of Tissue Inhibitor of Metalloproteinases-1

Entity

1. N-terminal domain of Tissue Inhibitor of Metalloproteinases-1 (polymer, Thiol state: all disulfide bound), 126 monomers, 14252.16 Da Detail

CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEE

Formula weight

14252.16 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS70:SG
2	disulfide	sing	1:CYS3:SG	1:CYS99:SG
3	disulfide	sing	1:CYS13:SG	1:CYS124:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS FOLLOWED BY CARTESIAN SIMULATED ANNEALING

Data set

heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters

Protein Blocks Logo

Calculated from 29 models in PDB: 1D2B, Strand ID: A Detail

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Heteronucl. T₁

102 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 500 MHz, Temperature 293 (±0.5) K, pH 6.0 (±0.05) Detail

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Temperature 293 (±0.5) K, pH 6.0 (±0.05)

Experiment name 15N T1 relaxation

Heteronucl. T₂

102 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, Temperature 293 (±0.5) K, pH 6.0 (±0.05) Detail

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Temperature 293 (±0.5) K, pH 6.0 (±0.05)

Experiment name 15N T2 relaxation

Heteronucl. NOE

102 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 500 MHz, Temperature 293 (±0.5) K, pH 6.0 (±0.05) Detail

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Temperature 293 (±0.5) K, pH 6.0 (±0.05)

Experiment name heteronuclear 1H-15N NOE

Heteronucl. T₁/T₂

102 T₁/T₂ values in 1 lists
Field strength (¹H) 500 MHz, Temperature 293 (±0.5) K, pH 6.0 (±0.05) Detail

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Temperature 293 (±0.5) K, pH 6.0 (±0.05)

Order parameters

102 S² values in 1 lists
Temperature 293 (±0.5) K, pH 6.0 (±0.05) Detail

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Temperature 293 (±0.5) K, pH 6.0 (±0.05)

Experiment name transverse cross-relaxation measurement

List #1 S2_parameters_label

Release date

2001-09-16

Citation

Tissue inhibitor of metalloproteinases-1 undergoes microsecond to millisecond motions at sites of matrix metalloproteinase-induced fit
Gao, G., Semenchenko, V., Arumugam, S., Van Doren, S.R.
J. Mol. Biol. (2000), 301, 537-552, PubMed 10926526 , DOI 10.1006/jmbi.2000.3976 , Abstract

Related entities 1. N-terminal domain of Tissue Inhibitor of Metalloproteinases-1, : 1 : 5 : 4 : 56 entities Detail

Interaction partners 1. N-terminal domain of Tissue Inhibitor of Metalloproteinases-1, : 6 interactors Detail

Experiments performed 4 experiments Detail