BMRB: 5157

Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein

Authors

Liepinsh, E., Otting, G., Harding, M.M., Ward, L.G., Mackay, J.P., Haymet, A.D.J.

Assembly

Antifreeze protein type 1 mutant

Entity

1. Antifreeze protein type 1 mutant (polymer, Thiol state: not present), 37 monomers, 3464.854 Da Detail

DVASDAKAAA ELVAANAKAA AELVAANAKA AAEAVAX

Formula weight

3464.854 Da

Source organism

Pseudopleuronectes americanus

Exptl. method

NMR

Refine. method

torsion angle dynamics,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 92.0 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	92.0 % (150 of 163)	92.0 % (150 of 163)
Backbone	98.6 % (71 of 72)	98.6 % (71 of 72)
Sidechain	86.8 % (79 of 91)	86.8 % (79 of 91)
Methyl	100.0 % (31 of 31)	100.0 % (31 of 31)

1. antifreeze protein type 1 mutant

DVASDAKAAA ELVAANAKAA AELVAANAKA AAEAVAX

Show sample condition

Sample

Temperature 283 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	antifreeze protein type 1 mutant			2 mM
4	NaCl			100 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1J5B, Strand ID: A Detail

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Release date

2008-07-15

Citation 1

Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein
Liepinsh, E., Otting, G., Harding, M.M., Ward, L.G., Mackay, J.P., Haymet, A.D.J.
Eur. J. Biochem. (2002), 269, 1259-1266, PubMed 11856360 , DOI: , Abstract

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Citation 2

Valine substituted winter flounder 'antifreeze': preservation of ice growth hysteresis
Haymet, A.D., Ward, L.G., Harding, M.M., Knight, C.A.
FEBS Lett. (1998), 430, 301-306, PubMed 9688560 , DOI: , Abstract

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Experiments performed 4 experiments Detail