BMRB: 5162

1H, 13C, and 15N resonance assignments and secondary structure of the PWI domain from SRm160 using Reduced Dimensionality NMR

Authors

Szymczyna, B.R., Pineda-Lucena, A., Mills, J.L., Szyperski, T., Arrowsmith, C.H.

Assembly

SRm160

Entity

1. SRm160 (polymer, Thiol state: all free), 109 monomers, 12628.70 Da Detail

MQLKFAECLE KKVDMSKVNL EVIKPWITKR VTEILGFEDD VVIEFIFNQL EVKNPDSKMM QINLTGFLNG KNAREFMGEL WPLLLSAQEN IAGIPSAFLE LKKEEIKQR

Formula weight

12628.7 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.1 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.1 % (1328 of 1367)	97.8 % (704 of 720)	96.2 % (508 of 528)	97.5 % (116 of 119)
Backbone	98.6 % (637 of 646)	99.5 % (218 of 219)	97.8 % (315 of 322)	99.0 % (104 of 105)
Sidechain	96.2 % (794 of 825)	97.0 % (486 of 501)	95.5 % (296 of 310)	85.7 % (12 of 14)
Aromatic	77.7 % (73 of 94)	80.9 % (38 of 47)	73.3 % (33 of 45)	100.0 % (2 of 2)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. SRm160

MQLKFAECLE KKVDMSKVNL EVIKPWITKR VTEILGFEDD VVIEFIFNQL EVKNPDSKMM QINLTGFLNG KNAREFMGEL WPLLLSAQEN IAGIPSAFLE LKKEEIKQR

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	SRm160	[U-15N; U-13C]		1.5 mM
2	sodium phosphate dibasic			7.9 mM
3	sodium phosphate monobasic			17.1 mM
4	sodium chloride			300 mM

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1MP1, Strand ID: A Detail

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Release date

2002-12-26

Citation

1H, 13C, and 15N resonance assignments and secondary structure of the PWI domain from SRm160 using reduced dimensionality NMR
Szymczyna, B.R., Pineda-Lucena, A., Mills, J.L., Szyperski, T., Arrowsmith, C.H.
J. Biomol. NMR (2002), 22, 299-300, PubMed 11991360 , DOI: , Abstract

Related entities 1. SRm160, : 1 : 6 : 15 entities Detail

Interaction partners 1. SRm160, : 9 interactors Detail