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BMRB: 5165


5165
1JW3
Solution Structure of Methanobacterium Thermoautotrophicum Protein 1598
Authors
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C.H., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Assembly
Conserved Hypothetical Protein MTH1598
Entity
1. Conserved Hypothetical Protein MTH1598 (polymer, Thiol state: not present), 140 monomers, 16183.09 Da Detail

MKGFEFFDVT ADAGFWAYGH DLEEVFENAA LAMFEVMTDT SLVEAAEERR VEITSEDRVS LLYDWLDELL FIHDTEFILF SKFKVKIDEK DDGLHLTGTA MGEEIKEGHE RRDEVKAVTF HMMEILDEDG LIKARVILDL


Formula weight
16183.09 Da
Source organism
Methanothermobacter thermautotrophicus
Exptl. method
NMR
Refine. method
distance geometry
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 89.1 %, Completeness (bb): 98.2 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All89.1 % (1467 of 1646)88.5 % (746 of 843)87.9 % (580 of 660)98.6 % (141 of 143)
Backbone98.2 % (825 of 840)98.6 % (284 of 288)97.6 % (402 of 412)99.3 % (139 of 140)
Sidechain82.3 % (772 of 938)83.2 % (462 of 555)81.1 % (308 of 380)66.7 % (2 of 3)
Aromatic50.6 % (86 of 170)58.8 % (50 of 85)41.0 % (34 of 83)100.0 % (2 of 2)
Methyl92.4 % (157 of 170)94.1 % (80 of 85)90.6 % (77 of 85)

1. single chain biopolymer

MKGFEFFDVT ADAGFWAYGH DLEEVFENAA LAMFEVMTDT SLVEAAEERR VEITSEDRVS LLYDWLDELL FIHDTEFILF SKFKVKIDEK DDGLHLTGTA MGEEIKEGHE RRDEVKAVTF HMMEILDEDG LIKARVILDL

Show sample condition
Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)


#NameIsotope labelingTypeConcentration
1single chain biopolymer[U-15N; U-13C]2 mM
2NaCl300 mM
3phosphate10 mM
4H2O90 %
5D2O10 %

LACS Plot; CA
Referencing offset: -0.38 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.38 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.54 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 1JW3, Strand ID: A Detail

Release date
2002-05-05
Citation
An NMR approach to structural proteomics
Yee, A., Chang, X., Pineda-Lucena, A., Wu, B., Semesi, A., Le, B., Ramelot, T., Lee, G.M., Bhattacharyya, S., Gutierrez, P., Denisov, A., Lee, C.H., Cort, J.R., Kozlov, G., Liao, J., Finak, G., Chen, L., Wishart, D., Lee, W., McIntosh, L.P., Gehring, K., Kennedy, M.A., Edwards, A.M., Arrowsmith, C.H.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 1825-1830, PubMed 11854485 , DOI 10.1073/pnas.042684599 ,
Related entities 1. Conserved Hypothetical Protein MTH1598, : 1 : 1 : 44 entities Detail
More details for 46 related entities
Experiments performed 3 experiments Detail
nullKeywords structural genomics