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Found 1 Document (1.303 seconds)

BMRB: 5170

1JU7

NMR Structure and Dynamics of the RNA Binding Site for the Histone mRNA Stem-Loop Binding Protein

Authors

DeJong, E., Marzluff, W.F., Nikonowicz, E.P.

Assembly

Histone mRNA

Entity

1. Histone mRNA (polymer, Thiol state: not present), 28 monomers, 8983.327 Da Detail

GGCCCUUUUC AGGGCCCAGG GCCACCCA

Formula weight

8983.327 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 57.1 %, Completeness: 53.3 %, Completeness (bb): 57.1 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	53.3 % (241 of 452)	54.0 % (136 of 252)	53.7 % (101 of 188)	33.3 % (4 of 12)
Suger, PO4	57.1 % (176 of 308)	57.1 % (96 of 168)	57.1 % (80 of 140)
Nucleobase	45.1 % (65 of 144)	47.6 % (40 of 84)	43.8 % (21 of 48)	33.3 % (4 of 12)
Aromatic	49.2 % (59 of 120)	56.7 % (34 of 60)	43.8 % (21 of 48)	33.3 % (4 of 12)

1. Histone mRNA

GGCCCUUUUC AGGGCCCAGG GCCACCCA

Show sample condition

Sample

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Histone mRNA	[U-13C]	2.3 mM
2	KPi		20 mM
3	KCl		20 mM
4	EDTA		0.02 mM
5	D2O		100 %

Release date

2002-05-05

Citation

NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop binding protein
DeJong, E.S., Marzluff, W.F., Nikonowicz, E.P.
RNA (2002), 8, 83-96, PubMed 11871662 , DOI: , Abstract

Experiments performed 5 experiments Detail

1 NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Histone mRNA	[U-13C]	2.3 mM
2	KPi		20 mM
3	KCl		20 mM
4	EDTA		0.02 mM
5	D2O		100 %

Sample #2

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	Histone mRNA	[U-15N]	2.5 mM
7	KPi		20 mM
8	KCl		20 mM
9	EDTA		0.02 mM
10	D2O		10 %
11	H2O		90 %

Sample #3

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
12	Histone mRNA	[U-13C], [U-2H]-H5',H5''	2.0 mM
13	KPi		20 mM
14	KCl		20 mM
15	EDTA		0.02 mM
16	D2O		100 %

Sample #4

Temperature 280 K, pH 6.8

#	Name	Concentration
17	Histone mRNA	2.8 mM
18	KPi	20 mM
19	KCl	20 mM
20	EDTA	0.02 mM
21	D2O	100 %

2 3D 13C-separated NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Histone mRNA	[U-13C]	2.3 mM
2	KPi		20 mM
3	KCl		20 mM
4	EDTA		0.02 mM
5	D2O		100 %

Sample #2

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	Histone mRNA	[U-15N]	2.5 mM
7	KPi		20 mM
8	KCl		20 mM
9	EDTA		0.02 mM
10	D2O		10 %
11	H2O		90 %

Sample #3

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
12	Histone mRNA	[U-13C], [U-2H]-H5',H5''	2.0 mM
13	KPi		20 mM
14	KCl		20 mM
15	EDTA		0.02 mM
16	D2O		100 %

Sample #4

Temperature 280 K, pH 6.8

#	Name	Concentration
17	Histone mRNA	2.8 mM
18	KPi	20 mM
19	KCl	20 mM
20	EDTA	0.02 mM
21	D2O	100 %

3 3D 15N-separated NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Histone mRNA	[U-13C]	2.3 mM
2	KPi		20 mM
3	KCl		20 mM
4	EDTA		0.02 mM
5	D2O		100 %

Sample #2

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	Histone mRNA	[U-15N]	2.5 mM
7	KPi		20 mM
8	KCl		20 mM
9	EDTA		0.02 mM
10	D2O		10 %
11	H2O		90 %

Sample #3

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
12	Histone mRNA	[U-13C], [U-2H]-H5',H5''	2.0 mM
13	KPi		20 mM
14	KCl		20 mM
15	EDTA		0.02 mM
16	D2O		100 %

Sample #4

Temperature 280 K, pH 6.8

#	Name	Concentration
17	Histone mRNA	2.8 mM
18	KPi	20 mM
19	KCl	20 mM
20	EDTA	0.02 mM
21	D2O	100 %

4 DQF-COSY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Histone mRNA	[U-13C]	2.3 mM
2	KPi		20 mM
3	KCl		20 mM
4	EDTA		0.02 mM
5	D2O		100 %

Sample #2

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	Histone mRNA	[U-15N]	2.5 mM
7	KPi		20 mM
8	KCl		20 mM
9	EDTA		0.02 mM
10	D2O		10 %
11	H2O		90 %

Sample #3

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
12	Histone mRNA	[U-13C], [U-2H]-H5',H5''	2.0 mM
13	KPi		20 mM
14	KCl		20 mM
15	EDTA		0.02 mM
16	D2O		100 %

Sample #4

Temperature 280 K, pH 6.8

#	Name	Concentration
17	Histone mRNA	2.8 mM
18	KPi	20 mM
19	KCl	20 mM
20	EDTA	0.02 mM
21	D2O	100 %

5 HetCor

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Histone mRNA	[U-13C]	2.3 mM
2	KPi		20 mM
3	KCl		20 mM
4	EDTA		0.02 mM
5	D2O		100 %

Sample #2

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	Histone mRNA	[U-15N]	2.5 mM
7	KPi		20 mM
8	KCl		20 mM
9	EDTA		0.02 mM
10	D2O		10 %
11	H2O		90 %

Sample #3

Temperature 280 K, pH 6.8

#	Name	Isotope labeling	Concentration
12	Histone mRNA	[U-13C], [U-2H]-H5',H5''	2.0 mM
13	KPi		20 mM
14	KCl		20 mM
15	EDTA		0.02 mM
16	D2O		100 %

Sample #4

Temperature 280 K, pH 6.8

#	Name	Concentration
17	Histone mRNA	2.8 mM
18	KPi	20 mM
19	KCl	20 mM
20	EDTA	0.02 mM
21	D2O	100 %

nullKeywords 3- stack, hairpin, tetraloop

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Found 1 Document (1.303 seconds)

BMRB: 5170

Sample

Experiments performed 5 experiments Detail

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