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Found 1 Document (2.88 seconds)

BMRB: 5177

1TVJ

Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for chick cofilin

Authors

Bains, N.P.S., Gorbatyuk, V.YA., Nosworthy, N.J., Robson, S.A., Maciejewski, M.W., dos Remedios, C.G., King, G.F.

Assembly

chick cofilin

Entity

1. chick cofilin (polymer, Thiol state: all free), 166 monomers, 18661.35 Da Detail

MASGVTVNDE VIKVFNDMKV RKSSTPEEIK KRKKAVLFCL SDDKKQIIVE EAKQILVGDI GDTVEDPYTA FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL

Formula weight

18661.35 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

torsion angle dynamics followed by simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.3 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.3 % (1873 of 1986)	97.8 % (1015 of 1038)	89.6 % (697 of 778)	94.7 % (161 of 170)
Backbone	96.2 % (947 of 984)	96.7 % (324 of 335)	96.5 % (472 of 489)	94.4 % (151 of 160)
Sidechain	93.2 % (1080 of 1159)	98.3 % (691 of 703)	85.0 % (379 of 446)	100.0 % (10 of 10)
Aromatic	53.1 % (68 of 128)	100.0 % (64 of 64)	3.2 % (2 of 62)	100.0 % (2 of 2)
Methyl	99.5 % (195 of 196)	99.0 % (97 of 98)	100.0 % (98 of 98)

1. chick cofilin

MASGVTVNDE VIKVFNDMKV RKSSTPEEIK KRKKAVLFCL SDDKKQIIVE EAKQILVGDI GDTVEDPYTA FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1TVJ, Strand ID: A Detail

Release date

2002-05-05

Citation 1

Backbone and side-chain 1H, 15N, and 13C assignments for chick cofilin
Bains, N.P.S., Gorbatyuk, V.YA., Nosworthy, N.J., Robson, S.A., Maciejewski, M.W., dos Remedios, C.G., King, G.F.
J. Biomol. NMR (2002), 22, 193-194, PubMed 11885570 , Abstract

Show more 2 citaions

Citation 2

Sequence of cDNAs encoding actin depolymerizing factor and cofilin of embryonic chicken skeletal muscle: two functionally distinct actin-regulatory proteins exhibit high structural homology
Abe, H., Endo, T., Yamamoto, K., Obinata, T.
Biochemistry (1990), 29, 7420-7425, PubMed 1699599 , DOI: , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 2 citaions

Related entities 1. chick cofilin, : 1 : 2 : 159 entities Detail

Interaction partners 1. chick cofilin, : 27 interactors Detail

More details for 27 interactors identified by 8 citations

Experiments performed 13 experiments Detail

1 3D HNCACB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

2 3D CBCA(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

3 3D HNCO

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

4 3D HCCH-COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

5 3D HCCH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

6 3D HC(CO)NH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

7 3D C(CO)NH-TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

8 3D 15N-edited NOESY-HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

9 3D 13C-edited NOESY-HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

10 2D (HB)CB(CGCD)HD

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

11 2D 1H-1H NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

12 2D 1H-1H DQF-COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

13 2D TOCSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	chick cofilin	[U-99% 15N]	1.0 mM
2	NaCl		50 mM
3	PIPES		10 mM
4	H2O		92.5 %
5	D2O		7.5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
6	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
7	NaCl		50 mM
8	PIPES		10 mM
9	H2O		92.5 %
10	D2O		7.5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
11	chick cofilin	[U-99% 13C; U-99% 15N]	1.0 mM
12	NaCl		50 mM
13	PIPES		10 mM
14	D2O		100 %

nullKeywords cofilin, actin

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Found 1 Document (2.88 seconds)

BMRB: 5177

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. chick cofilin, : 1 : 2 : 159 entities Detail

Interaction partners 1. chick cofilin, : 27 interactors Detail

Experiments performed 13 experiments Detail

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