BMRBj - BMREntryMaster

Found 1 Document (1.816 seconds)

BMRB: 5180

1HBW

Solution nmr structure of the dimerization domain of the yeast transcriptional activator Gal4 (residues 50-106)

Authors

Hidalgo, P., Ansari, A.Z., Schmidt, P., Hare, B., Simkovic, N., Farrell, S., Shin, E.J., Ptashne, M., Wagner, G.

Assembly

GAL4 dimerization domain

Entity

1. GAL4 dimerization domain (polymer, Thiol state: not present), 57 monomers, 6685.635 × 2 Da Detail

TRAHLTEVES RLERLEQLFL LIFPREDLDM ILKMDSLRDI EALLTGLFVQ DNVNKDA

Total weight

13371.27 Da

Max. entity weight

6685.635 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING PROTOCOL

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 75.4 %, Completeness: 33.9 %, Completeness (bb): 45.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.9 % (234 of 690)	40.9 % (147 of 359)	17.7 % (48 of 271)	65.0 % (39 of 60)
Backbone	45.9 % (156 of 340)	66.7 % (76 of 114)	24.1 % (41 of 170)	69.6 % (39 of 56)
Sidechain	20.4 % (83 of 406)	29.0 % (71 of 245)	7.6 % (12 of 157)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 34)	0.0 % (0 of 17)	0.0 % (0 of 17)
Methyl	19.0 % (16 of 84)	28.6 % (12 of 42)	9.5 % (4 of 42)

1. GAL4 dimerization domain

TRAHLTEVES RLERLEQLFL LIFPREDLDM ILKMDSLRDI EALLTGLFVQ DNVNKDA

Show sample condition

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

Protein Blocks Logo

Calculated from 17 models in PDB: 1HBW, Strand ID: A, B Detail

Release date

2001-11-11

Citation

Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain
Hidalgo, P., Ansari, A.Z., Schmidt, P., Hare, B., Simkovic, N., Farrell, S., Shin, E.J., Ptashne, M., Wagner, G.
Genes. Dev. (2001), 15, 1007-1020, PubMed 11316794 , DOI 10.1101/gad.873901 , Abstract

Related entities 1. GAL4 dimerization domain, : 1 : 2 : 3 entities Detail

Interaction partners 1. GAL4 dimerization domain, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 9 experiments Detail

1 2D 1H-1H NOESY

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

2 2D 1H-13C HSQC

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

3 2D 1H-15N HSQC

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

4 3D 1H-1H-15N NOESY

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

5 3D 13C-1H-1H NOESY

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

6 3D HNCA

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

7 3D HN(CO)CA

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

8 3D HNHA

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

9 3D HNHB

Instrument

#1 Model Varian UnityPlus (400 MHz)

#2 Model Varian Unity (500 MHz)

#3 Model Varian INOVA (500 MHz)

#4 Model Varian UnityPlus (750 MHz)

#5 Model Bruker AMX (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
1	GAL4 dimerization domain			1.0 ~ 2.0 mM

Sample #2

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
2	GAL4 dimerization domain	[U-15N; U-2H]		1.0 ~ 2.0 mM

Sample #3

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
3	GAL4 dimerization domain	[U-15N]		1.0 ~ 2.0 mM

Sample #4

Temperature 308 (±1) K, pH 7.4 (±0.2), Details Dimer concentration is 0.5 - 1 mM

#	Name	Isotope labeling	Type	Concentration
4	GAL4 dimerization domain	[U-15N; U-13C]		1.0 ~ 2.0 mM

nullKeywords coiled-coil homodimer, dimerization domain, galactose and melibiose metabolism, Transcriptional activator

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Found 1 Document (1.816 seconds)

BMRB: 5180

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Related entities 1. GAL4 dimerization domain, : 1 : 2 : 3 entities Detail

Interaction partners 1. GAL4 dimerization domain, : 2 interactors Detail

Experiments performed 9 experiments Detail

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