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BMRB: 5181

1K42

Virtually complete 1H, 13C and 15N resonance assignments of the second family 4 xylan binding module of Rhodothermus marinus xylanase 10A

Authors

Jamieson, S.J., Williamson, M.P., Abou-Hachem, M., Nordberg Karlsson, E., Simpson, P.J.

Assembly

Xylanase 10A, second family 4 carbohydrate binding module

Entity

1. Xylanase 10A, second family 4 carbohydrate binding module (polymer, Thiol state: not present), 168 monomers, 18096.72 Da Detail

MLVANINGGF ESTPAGVVTD LAEGVEGWDL NVGSSVTNPP VFEVLETSDA PEGNKVLAVT VNGVGNNPWD IEATAFPVNV RPGVTYTYTI WARAEQDGAV VSFTVGNQSF QEYGRLHEQQ ITTEWQPFTF EFTVSDQETV IRAPIHFGYA ANVGNTIYID GLAIASQP

Formula weight

18096.72 Da

Source organism

Rhodothermus marinus

Exptl. method

NMR

Refine. method

hybrid distance geometry/simulated annealing using XPLOR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.0 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.0 % (1808 of 1863)	96.9 % (915 of 944)	96.7 % (713 of 737)	98.9 % (180 of 182)
Backbone	99.1 % (979 of 988)	99.1 % (339 of 342)	99.2 % (484 of 488)	98.7 % (156 of 158)
Sidechain	95.4 % (980 of 1027)	95.7 % (576 of 602)	94.8 % (380 of 401)	100.0 % (24 of 24)
Aromatic	82.3 % (153 of 186)	82.8 % (77 of 93)	80.9 % (72 of 89)	100.0 % (4 of 4)
Methyl	100.0 % (210 of 210)	100.0 % (105 of 105)	100.0 % (105 of 105)

1. cbm4-2

MLVANINGGF ESTPAGVVTD LAEGVEGWDL NVGSSVTNPP VFEVLETSDA PEGNKVLAVT VNGVGNNPWD IEATAFPVNV RPGVTYTYTI WARAEQDGAV VSFTVGNQSF QEYGRLHEQQ ITTEWQPFTF EFTVSDQETV IRAPIHFGYA ANVGNTIYID GLAIASQP

Show sample condition

Sample

Temperature 313 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	cbm4-2	[U-13C; U-15N]	1.5 mM
2	CaCl2		50 mM
3	sodium acetate	[U-2H]	50 mM
4	sodium azide		10 mM
5	TSP		0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291
¹³C	TSP	methyl protons	null	indirect	0.2514495

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291
¹³C	TSP	methyl protons	null	indirect	0.2514495

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291
¹³C	TSP	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	null	indirect	0.1013291
¹³C	TSP	methyl protons	null	indirect	0.2514495

Referencing offset: 0.75 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1K45, Strand ID: A Detail

Release date

2002-05-06

Citation

Virtually complete 1H, 13C and 15N resonance assignments of the second family 4 xylan binding module of Rhodothermus marinus xylanase 10A
Jamieson, S.J., Williamson, M.P., Abou-Hachem, M., Nordberg, K.E., Simpson, P.J.
J. Biomol. NMR (2002), 22, 187-188, PubMed 11883782 , DOI: , Abstract

Related entities 1. Xylanase 10A, second family 4 carbohydrate binding module, : 1 : 1 : 12 entities Detail

Experiments performed 12 experiments Detail

1 HNHA