BMRB: 5187

Assignments of the 1H, 13C, and 15N resonances of TraR

Authors

Tanaka, T., Sugai, M., Kobayashi, K., Kataoka, M., Kohno, T.

Assembly

TraR monomer

Entity

1. TraR monomer (polymer, Thiol state: not present), 102 monomers, 10710.24 Da Detail

GPMPYKAPEG KGYADVATHF RTLIKSGELA PGDTLPSVAD IRAQFGVAAK TVSRALAVLK SEGLVSSRGA LGTVVEKNPI VITGADRLKR MEKNGMRYAP GE

Formula weight

10710.24 Da

Source organism

Streptomyces flavovirens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics, matrix relaxation, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.5 %, Completeness (bb): 97.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.5 % (952 of 1126)	80.0 % (468 of 585)	88.3 % (391 of 443)	94.9 % (93 of 98)
Backbone	97.7 % (584 of 598)	95.7 % (200 of 209)	99.0 % (291 of 294)	97.9 % (93 of 95)
Sidechain	74.8 % (462 of 618)	72.1 % (271 of 376)	79.9 % (191 of 239)	0.0 % (0 of 3)
Aromatic	16.7 % (8 of 48)	16.7 % (4 of 24)	16.7 % (4 of 24)
Methyl	85.2 % (104 of 122)	91.8 % (56 of 61)	78.7 % (48 of 61)

1. Streptmycal repressor

GPMPYKAPEG KGYADVATHF RTLIKSGELA PGDTLPSVAD IRAQFGVAAK TVSRALAVLK SEGLVSSRGA LGTVVEKNPI VITGADRLKR MEKNGMRYAP GE

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Streptmycal repressor	[U-99% 13C; U-99% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
2H	DSS	methyl protons	0.0 ppm	null	indirect	0.1535061
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
2H	DSS	methyl protons	0.0 ppm	null	indirect	0.1535061
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
2H	DSS	methyl protons	0.0 ppm	null	indirect	0.1535061
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
2H	DSS	methyl protons	0.0 ppm	null	indirect	0.1535061
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 1V4R, Strand ID: A Detail

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Release date

2002-08-21

Citation

Complete 1H, 13C, and 15N assignments of the N-terminal DNA binding domain of the TraR protein
Tanaka, T., Sugai, M., Kobayashi, K., Kataoka, M., Kohno, T.
J. Biomol. NMR (2002), 23, 161-162, PubMed 12153045 , DOI: , Abstract

Related entities 1. TraR monomer, : 1 : 38 entities Detail

Experiments performed 12 experiments Detail