BMRB: 5193

The solution structure of the mutant 5'AUG3' triloop in the RNA promoter region of the brome mosaic virus genomic (+)-RNA

Authors

Kim, C., Kao, C.C.

Assembly

5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3'

Entity

1. 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3' (polymer, Thiol state: not present), 13 monomers, 4235.514 Da Detail

GGUGCAUGGC ACC

Formula weight

4235.514 Da

Source organism

Brome mosaic virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 7.7 %, Completeness: 7.0 %, Completeness (bb): 7.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polyribonucleotide

	Total	1H
All	7.0 % (8 of 114)	7.0 % (8 of 114)
Suger, PO4	7.7 % (6 of 78)	7.7 % (6 of 78)
Nucleobase	5.6 % (2 of 36)	5.6 % (2 of 36)
Aromatic	7.1 % (2 of 28)	7.1 % (2 of 28)

1. 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3'

GGUGCAUGGC ACC

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3'			2 mM
2	sodium phosphate			10 mM
3	NaCl			10 mM

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 7.7 %, Completeness: 7.0 %, Completeness (bb): 7.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polyribonucleotide

	Total	1H
All	7.0 % (16 of 228)	7.0 % (16 of 228)
Suger, PO4	7.7 % (12 of 156)	7.7 % (12 of 156)
Nucleobase	5.6 % (4 of 72)	5.6 % (4 of 72)
Aromatic	7.1 % (4 of 56)	7.1 % (4 of 56)

1. 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3'

GGUGCAUGGC ACC

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3'			2 mM
2	sodium phosphate			10 mM
3	NaCl			10 mM

Hide sample codition

Release date

2003-01-05

Citation

A mutant viral RNA promoter with an altered conformation retains efficient recognition by a viral RNA replicase through a solution-exposed adenine
Kim, C., Kao, C.C.
RNA (2001), 7, 1476-1485, PubMed 11680852 , DOI: , Abstract

Related entities 1. 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3', : 1 : 1 entities Detail

Experiments performed 2 experiments Detail